NDLI: Expression of human adenosine deaminase in murine hematopoietic cells.

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Expression of human adenosine deaminase in murine hematopoietic cells.

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Murine Hox-1.7 Homeo-Box Gene: Cloning, Chromosomal Location, and Expression

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Expression of human adenosine deaminase in murine hematopoietic cells.

Content Provider	PubMed Central
Author	Belmont, J. W. Macgregor, G. R. Wager-smith, K. Fletcher, F. A. Moore, K. A. Hawkins, D. Villalon, D. Chang, S. M. Caskey, C. T.
Abstract	Multiple replication-defective retrovirus vectors were tested for their ability to transfer and express human adenosine deaminase in vitro and in vivo in a mouse bone marrow transplantation model. High-titer virus production was obtained from vectors by using both a retrovirus long terminal repeat promoter and internal transcriptional units with human c-fos and herpes virus thymidine kinase promoters. After infection of primary murine bone marrow with one of these vectors, human adenosine deaminase was detected in 60 to 85% of spleen colony-forming units and in the blood of 14 of 14 syngeneic marrow transplant recipients. This system offers the opportunity to assess methods for increasing efficiency of gene transfer, for regulation of expression of foreign genes in hematopoietic progenitors, and for long-term measurement of the stability of expression in these cells.
Starting Page	5116
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	12
Volume Number	8
Language	English
Publisher Date	1988-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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