NDLI: Multiple subelements within the polyomavirus enhancer function synergistically to activate DNA replication.

Please wait, while we are loading the content...

Year: 2016, Volume: 36

Year: 2015, Volume: 36

Year: 2015, Volume: 35

Year: 2014, Volume: 35

Year: 2014, Volume: 34

Year: 2013, Volume: 33

Year: 2012, Volume: 32

Year: 2011, Volume: 31

Year: 2010, Volume: 30

Year: 2009, Volume: 29

Year: 2008, Volume: 28

Year: 2007, Volume: 27

Year: 2006, Volume: 26

Year: 2005, Volume: 25

Year: 2004, Volume: 24

Year: 2003, Volume: 23

Year: 2002, Volume: 23

Year: 2002, Volume: 22

Year: 2001, Volume: 21

Year: 2000, Volume: 20

Year: 1999, Volume: 19

Year: 1998, Volume: 18

Year: 1997, Volume: 17

Year: 1996, Volume: 16

Year: 1995, Volume: 15

Year: 1994, Volume: 14

Year: 1993, Volume: 13

Year: 1992, Volume: 12

Year: 1991, Volume: 11

Year: 1990, Volume: 10

Year: 1989, Volume: 9

Year: 1988, Volume: 8

Year: 1988, Volume: 8, Issue: 12

Year: 1988, Volume: 8, Issue: 11

The chicken skeletal alpha-actin gene promoter region exhibits partial dyad symmetry and a capacity to drive bidirectional transcription.

DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context.

Transcriptional regulation of the cell cycle-dependent thymidylate synthase gene of Saccharomyces cerevisiae.

Posttranscriptional regulation of cytochrome c expression during the developmental cycle of Trypanosoma brucei.

A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box.

Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae.

ROX1 encodes a heme-induced repression factor regulating ANB1 and CYC7 of Saccharomyces cerevisiae.

Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts.

Extensive movement of LINES ONE sequences in beta-globin loci of Mus caroli and Mus domesticus.

DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.

Increased amount of a 25-kilodalton phosphoprotein after v-mos transfection of CHO cells.

A short-lived nuclear phosphoprotein encoded by the human ets-2 proto-oncogene is stabilized by activation of protein kinase C.

Two regulatory domains flank the mouse H19 gene.

Coordinate induction of metallothioneins I and II in rodent cells by UV irradiation.

Assignment of cloned genes to the seven electrophoretically separated Candida albicans chromosomes.

Structure and expression of ubiquitin genes of Drosophila melanogaster.

Coordinate changes in heat shock element-binding activity and HSP70 gene transcription rates in human cells.

Tandemly repeated exons encode 81-base repeats in multiple, developmentally regulated Schistosoma mansoni transcripts.

Sex-specific control of Drosophila melanogaster yolk protein 1 gene expression is limited to transcription.

Autonomous replication and addition of telomerelike sequences to DNA microinjected into Paramecium tetraurelia macronuclei.

Inositol regulates phosphatidylglycerolphosphate synthase expression in Saccharomyces cerevisiae.

Localization and expression of mRNA for a macronuclear-specific histone H2A variant (hv1) during the cell cycle and conjugation of Tetrahymena thermophila.

Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.

Gene dosage alteration of L2 ribosomal protein genes in Saccharomyces cerevisiae: effects on ribosome synthesis.

Concentration dependence of transcriptional transactivation in inducible E1A-containing human cells.

Molecular analysis of GCN3, a translational activator of GCN4: evidence for posttranslational control of GCN3 regulatory function.

Patterns of polyadenylation site selection in gene constructs containing multiple polyadenylation signals.

Rapid transcriptional regulation by phytochrome of the genes for phytochrome and chlorophyll a/b-binding protein in Avena sativa.

Interactions between the promoter and first intron are involved in transcriptional control of alpha 1(I) collagen gene expression.

Regulation of Rous sarcoma virus RNA splicing and stability.

Proviral insertional activation of c-erbB: differential processing of the protein products arising from two alternate transcripts.

Alpha-cell-specific expression of the glucagon gene is conferred to the glucagon promoter element by the interactions of DNA-binding proteins.

Organization of the Ly-5 gene.

c-kit protein, a transmembrane kinase: identification in tissues and characterization.

Intracellular precursors and secretion of alkaline extracellular protease of Yarrowia lipolytica.

Developmental programs of human erythroleukemia cells: globin gene expression and methylation.

Organization of replication of ribosomal DNA in Saccharomyces cerevisiae.

Copy number and partition of the Saccharomyces cerevisiae 2 micron plasmid controlled by transcription regulators.

The coordinate replication of the human beta-globin gene domain reflects its transcriptional activity and nuclease hypersensitivity.

Positional and developmental regulation of glutamine synthetase expression in mouse liver.

Purified mu EBP-E binds to immunoglobulin enhancers and promoters.

Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II.

GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae.

Multiple subelements within the polyomavirus enhancer function synergistically to activate DNA replication.

Gene expression of human DNA polymerase alpha during cell proliferation and the cell cycle.

Differential processing of colony-stimulating factor 1 precursors encoded by two human cDNAs.

Expression and processing of a recombinant human macrophage colony-stimulating factor in mouse cells.

Constitutive binding of yeast heat shock factor to DNA in vivo.

Reproducible and variable rearrangements of a Tetrahymena thermophila surface protein gene family occur during macronuclear development.

A silencer element from the alpha-globin gene inhibits expression of beta-like genes.

Platelet-derived growth factor does not induce c-fos in NIH 3T3 cells expressing the EJ-ras oncogene.

Effect of sequence differences between somatic and oocyte 5S RNA genes on transcriptional efficiency in an oocyte S150 extract.

Year: 1988, Volume: 8, Issue: 10

Year: 1988, Volume: 8, Issue: 9

Year: 1988, Volume: 8, Issue: 8

Year: 1988, Volume: 8, Issue: 7

Year: 1988, Volume: 8, Issue: 6

Year: 1988, Volume: 8, Issue: 5

Year: 1988, Volume: 8, Issue: 4

Year: 1988, Volume: 8, Issue: 3

Year: 1988, Volume: 8, Issue: 2

Year: 1988, Volume: 8, Issue: 1

Year: 1987, Volume: 7

Year: 1986, Volume: 6

Year: 1985, Volume: 5

Year: 1984, Volume: 4

Year: 1983, Volume: 3

Year: 1982, Volume: 2

Year: 1981, Volume: 1

Multiple subelements within the polyomavirus enhancer function synergistically to activate DNA replication.

Content Provider	PubMed Central
Author	Muller, W. J. Dufort, D. Hassell, J. A.
Abstract	The polyomavirus origin for DNA replication comprises at least two essential, but functionally distinct, cis-acting components. One of these, the origin core, is required only for DNA replication. It includes binding sites for large T antigen and the origin of bidirectional DNA replication. The other component is required for both transcription and DNA replication and is represented by two functionally redundant regions, alpha and beta, which are elements of the polyomavirus enhancer. Whereas either enhancer element will activate DNA replication, both enhancer elements are required to constitute a functional enhancer of transcription. To identify the sequences that make up each enhancer element, we have subjected them separately to in vitro mutagenesis and measured their capacity to activate replication in cis of the origin core in MOP-8 cells, which provide all trans-acting replicative functions including large T antigen. The results reveal that the beta enhancer element is composed of three subelements, two auxiliary subelements, and a core subelement. The core subelement independently activated DNA replication, albeit poorly. The auxiliary subelements, which were inactive on their own, acted synergistically with the core subelement to increase its activity. Interestingly, dimers of the beta core subelement functioned as well as the combination of a beta auxiliary subelement and a core subelement, suggesting that the subelements are functionally equivalent. The alpha enhancer element is organized similarly; it too comprises an auxiliary subelement and a core subelement. These results lead us to suggest that the polyomavirus enhancer comprises two levels of organization; two or more enhancer elements form an enhancer, and two or more subelements make up an enhancer element. The subelements share few sequences and serve as binding sites for distinct cellular factors. It appears, therefore, that a number of different cellular proteins function cooperatively to activate polyomavirus DNA replication by a common mechanism.
Starting Page	5000
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	11
Volume Number	8
Language	English
Publisher Date	1988-11-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in