NDLI: A synthetic silencer mediates SIR-dependent functions in Saccharomyces cerevisiae.

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Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia.

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Functional expression and RNA binding analysis of the interferon-induced, double-stranded RNA-activated, 68,000-Mr protein kinase in a cell-free system.

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Sequences and factors required for the F9 embryonal carcinoma stem cell E1a-like activity.

Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini.

Identification of a conserved lipopolysaccharide-plus-interleukin-4-responsive element located at the promoter of germ line epsilon transcripts.

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U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction.

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Id proteins Id1 and Id2 selectively inhibit DNA binding by one class of helix-loop-helix proteins.

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The intron-containing hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum is properly spliced in severe heat shock conditions.

Transcriptional activation through ETS domain binding sites in the cytochrome c oxidase subunit IV gene.

The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae.

A synthetic silencer mediates SIR-dependent functions in Saccharomyces cerevisiae.

Nonhomologous recombination at sites within the mouse JH-C delta locus accompanies C mu deletion and switch to immunoglobulin D secretion.

Identification of an essential site for transcriptional activation within the human T-cell receptor delta enhancer.

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.

AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae.

Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans.

Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae.

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Structural and functional dissection of a membrane glycoprotein required for vesicle budding from the endoplasmic reticulum.

nit-4, a pathway-specific regulatory gene of Neurospora crassa, encodes a protein with a putative binuclear zinc DNA-binding domain.

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CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase.

Mutations in a conserved region of RNA polymerase II influence the accuracy of mRNA start site selection.

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.

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A synthetic silencer mediates SIR-dependent functions in Saccharomyces cerevisiae.

Content Provider	PubMed Central
Author	Mcnally, F. J. Rine, J.
Abstract	Copies of the mating-type genes are present at three loci on chromosome III of the yeast Saccharomyces cerevisiae. The genes at the MAT locus are transcribed, whereas the identical genes at the silent loci, HML and HMR, are not transcribed. Several genes, including the four SIR genes, and two sites, HMR-E and HMR-I, are required for repression of transcription at the HMR locus. Three elements have been implicated in the function of the HMR-E silencer: a binding site for the RAP1 protein, a binding site for the ABF1 protein, and an 11-bp consensus sequence common to nearly all autonomously replicating sequence (ARS) elements (putative origins of DNA replication). RAP1 and ABF1 binding sites of different sequence than those found at HMR-E were joined with an 11-bp ARS consensus sequence to form a synthetic silencer. The synthetic silencer was able to repress transcription of the HMRa1 gene, confirming that binding sites for RAP1 and ABF1 and the 11-bp ARS consensus sequence were the functional components of the silencer in vivo. Mutations in the ABF1 binding site or in the ARS consensus sequence of the synthetic silencer caused nearly complete derepression of transcription at HMR. The ARS consensus sequence mutation also eliminated the ARS activity of the synthetic silencer. These data suggested that replication initiation at the HMR-E silencer was required for establishment of the repressed state at the HMR locus.
Starting Page	5648
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	11
Volume Number	11
Language	English
Publisher Date	1991-11-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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