NDLI: Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair.

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Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair.

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The rad16 gene of Schizosaccharomyces pombe: a homolog of the RAD1 gene of Saccharomyces cerevisiae.

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Growth hormone and erythropoietin differentially activate DNA-binding proteins by tyrosine phosphorylation.

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Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair.

Content Provider	PubMed Central
Author	Nassif, N. Penney, J. Pal, S. Engels, W. R. Gloor, G. B.
Abstract	P-element-induced gap repair was used to copy nonhomologous DNA into the Drosophila white locus. We found that nearly 8,000 bp of nonhomologous sequence could be copied from an ectopic template at essentially the same rate as a single-base substitution at the same location. An in vitro-constructed deletion was also copied into white at high frequencies. This procedure can be applied to the study of gene expression in Drosophila melanogaster, especially for genes too large to be manipulated in other ways. We also observed several types of more complex events in which the copied template sequences were rearranged such that the breakpoints occurred at direct duplications. Most of these can be explained by a model of double strand break repair in which each terminus of the break invades a template independently and serves as a primer for DNA synthesis from it, yielding two overlapping single-stranded sequences. These single strands then pair, and synthesis is completed by each using the other as a template. This synthesis-dependent strand annealing (SDSA) model as a possible general mechanism in complex organisms is discussed.
Starting Page	1613
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	3
Volume Number	14
Language	English
Publisher Date	1994-03-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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