NDLI: Efficient Repair of Abasic Sites in DNA by Mitochondrial Enzymes

Content Provider	PubMed Central
Author	Pinz, Kevin G. Bogenhagen, Daniel F.
Copyright Year	1998
Abstract	Mutations in mitochondrial DNA (mtDNA) cause a variety of relatively rare human diseases and may contribute to the pathogenesis of other, more common degenerative diseases. This stimulates interest in the capacity of mitochondria to repair damage to mtDNA. Several recent studies have shown that some types of damage to mtDNA may be repaired, particularly if the lesions can be processed through a base excision mechanism that employs an abasic site as a common intermediate. In this paper, we demonstrate that a combination of enzymes purified from Xenopus laevis mitochondria efficiently repairs abasic sites in DNA. This repair pathway employs a mitochondrial class II apurinic/apyrimidinic (AP) endonuclease to cleave the DNA backbone on the 5′ side of an abasic site. A deoxyribophosphodiesterase acts to remove the 5′ sugar-phosphate residue left by AP endonuclease. mtDNA polymerase γ fills the resulting 1-nucleotide gap. The remaining nick is sealed by an mtDNA ligase. We report the first extensive purification of mtDNA ligase as a 100-kDa enzyme that functions with an enzyme-adenylate intermediate and is capable of ligating oligo(dT) strands annealed to poly(rA). These properties together with preliminary immunological evidence suggest that mtDNA may be related to nuclear DNA ligase III.
Starting Page	1257
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	3
Volume Number	18
Language	English
Publisher	American Society for Microbiology
Publisher Date	1998-03-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Amino Acid Templating Mechanisms in Selection of Nucleotides Opposite Abasic Sites by a Family A DNA Polymerase

Repair of abasic sites by mammalian cell extracts.

Abasic Sites in the Transcribed Strand of Yeast DNA Are Removed by Transcription-Coupled Nucleotide Excision Repair▿ †

Human DNA Polymerase β Mutations Allowing Efficient Abasic Site Bypass

Translesion Synthesis of Abasic Sites by Yeast DNA Polymerase ϵ

Base Excision Repair Enzymes Protect Abasic Sites in Duplex DNA from Interstrand Cross-Links

A Versatile New Tool to Quantify Abasic Sites in DNA and Inhibit Base Excision Repair

Translesion Synthesis of Abasic Sites by Yeast DNA Polymerase ϵ*

Origin of Endogenous DNA Abasic Sites in Saccharomyces cerevisiae

Efficient Repair of Abasic Sites in DNA by Mitochondrial Enzymes

Similar Documents

Amino Acid Templating Mechanisms in Selection of Nucleotides Opposite Abasic Sites by a Family A DNA Polymerase

Repair of abasic sites by mammalian cell extracts.

Abasic Sites in the Transcribed Strand of Yeast DNA Are Removed by Transcription-Coupled Nucleotide Excision Repair▿ †

Human DNA Polymerase β Mutations Allowing Efficient Abasic Site Bypass

Translesion Synthesis of Abasic Sites by Yeast DNA Polymerase ϵ

Base Excision Repair Enzymes Protect Abasic Sites in Duplex DNA from Interstrand Cross-Links

A Versatile New Tool to Quantify Abasic Sites in DNA and Inhibit Base Excision Repair

Translesion Synthesis of Abasic Sites by Yeast DNA Polymerase ϵ*

Origin of Endogenous DNA Abasic Sites in Saccharomyces cerevisiae

Efficient Repair of Abasic Sites in DNA by Mitochondrial Enzymes