NDLI: Hydroxyapatite bioactivated bacterial cellulose promotes osteoblast growth and the formation of bone nodules

Content Provider	PubMed Central
Author	Tazi, Neftaha Zhang, Ze Younès, Messaddeq Almeida-lopes, Luciana Zanardi, Lisinéia M. Levinson, Dennis Rouabhia, Mahmoud
Copyright Year	2012
Abstract	The goal of this study was to investigate the feasibility of bacterial cellulose (BC) scaffold to support osteoblast growth and bone formation. BC was produced by culturing Acetobacter xylinum supplemented with hydroxyapatite (HA) to form BC membranes (without HA) and BC/HA membranes. Membranes were subjected to X-ray photoelectron spectroscopy (XPS) analysis to determine surface element composition. The membranes were further used to evaluate osteoblast growth, alkaline phosphatase activity and bone nodule formation. BC was free of calcium and phosphate. However, XPS analysis revealed the presence of both calcium (10%) and phosphate (10%) at the surface of the BC/HA membrane. Osteoblast culture showed that BC alone was non-toxic and could sustain osteoblast adhesion. Furthermore, osteoblast adhesion and growth were significantly (p ≤0.05) increased on BC/HA membranes as compared to BC alone. Both BC and BC/HA membranes improved osteoconductivity, as confirmed by the level of alkaline phosphatase (ALP) activity that increased from 2.5 mM with BC alone to 5.3 mM with BC/HA. BC/HA membranes also showed greater nodule formation and mineralization than the BC membrane alone. This was confirmed by Alizarin red staining (ARS) and energy dispersive X-ray spectroscopy (EDX). This work demonstrates that both BC and BC/HA may be useful in bone tissue engineering.
Related Links	http://dx.doi.org/10.1186/2191-0855-2-61
Ending Page	61
Page Count	1
Starting Page	61
File Format	PDF
ISSN	21910855
e-ISSN	21910855
Journal	AMB Express
Volume Number	2
Language	English
Publisher	Springer
Publisher Date	2012-11-22
Access Restriction	Open
Rights Holder	Springer
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics Applied Microbiology and Biotechnology

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