NDLI: Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1

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Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1

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Year: 2011, Volume: 1

Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1

Content Provider	PubMed Central
Author	Zhu, Xu-fen Tan, Hai-qin Zhu, Chu Liao, Li Zhang, Xin-qi Wu, Min
Copyright Year	2012
Abstract	A chitosanase gene, csn, was cloned from Penicillium sp. D-1 by inverse PCR. The cDNA sequence analysis revealed that csn had no intron. The deduced CSN protein consists of 250 amino acids including a 20-amino acid signal peptide, and shared 83.6% identity with the family 75 chitosanase from Talaromyces stipitatus (B8M2R4). The mature protein was overexpressed in Escherichia coli and purified with the affinity chromatography of Ni2+-NTA. The novel recombinant chitosanase showed maximal catalytic activity at pH 4.0 and 48°C. Moreover, the activity of CSN was stable over a broad pH range of 3.0-8.0, and the enzymatic activity was significantly enhanced by Mg2+ and Mn2+. The CSN could effectively hydrolyze colloidal chitosan and chitosan, while could not hydrolyze chitin and carboxymethylcellulose (CMC). Due to the particular acidophily, CSN has the potential application in the recycling of chitosan wastes.
Related Links	http://dx.doi.org/10.1186/2191-0855-2-13
Starting Page	13
File Format	PDF
ISSN	21910855
e-ISSN	21910855
Journal	AMB Express
Volume Number	2
Language	English
Publisher	Springer
Publisher Date	2012-01-01
Access Restriction	Open
Rights Holder	Springer
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics Applied Microbiology and Biotechnology

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