NDLI: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.

Please wait, while we are loading the content...

Year: 2016, Volume: 16

Year: 2015, Volume: 15

Year: 2014, Volume: 14

Year: 2013, Volume: 13

Year: 2013, Volume: 12

Year: 2012, Volume: 12

Year: 2011, Volume: 11

Year: 2010, Volume: 10

Year: 2009, Volume: 9

Year: 2008, Volume: 8

Year: 2007, Volume: 7

Year: 2006, Volume: 6

Year: 2005, Volume: 5

Year: 2005, Volume: 4

Year: 2004, Volume: 4

Year: 2003, Volume: 3

Year: 2002, Volume: 2

Year: 2002, Volume: 2, Issue: Unspecified

Methods for Maintaining Insect Cell Cultures

Characterization of Z/E11- and Z9-desaturases from the obliquebanded leafroller moth, Choristoneura rosaceana

The Natural History of the Arboreal Ant, Crematogaster ashmeadi

Karyotype, C- and G-band Patterns and DNA content of Callimenus (=Bradyporus) macrogaster macrogaster

Behavioral responses of adult female tobacco hornworms, Manduca sexta, to hostplant volatiles change with age and mating status

Substrate vibrations during acoustic signalling in the cicada Okanagana rimosa

The nest as fortress: defensive behavior of Polybia emaciata, a mud-nesting eusocial wasp

The protective nature of Chironomus luridus larval tubes against copper sulfate.

Fourth International Symposium on Molecular Insect Science

Zinc and Manduca sexta hemocyte functions

Ecdysteroids, Juvenile Hormone and Vitellogenesis in the Cockroach Leucophaea maderae.

Ecdysteroid 7,9(11)-dien-6-ones as potential photoaffinity labels for ecdysteroid binding proteins

Effects of juvenile hormone and ecdysone on the timing of vitellogenin appearance in hemolymph of queen and worker pupae of Apis mellifera

Aedes aegypti ferritin heavy chain homologue: feeding of iron or blood influences message levels, lengths and subunit abundance

Discontinuous gas exchange in a tracheate arthropod, the pseudoscorpion Garypus californicus: Occurrence, characteristics and temperature dependence.

Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.

The presence of the coffee berry borer, Hypothenemus hampei, in Puerto Rico: fact or fiction?

Year: 2001, Volume: 1

Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.

Content Provider	PubMed Central
Author	Lawrence, Pauline O.
Copyright Year	2002
Abstract	An insect poxvirus [entomopoxvirus (EPV)] occurs in the poison gland apparatus of female Diachasmimorpha longicaudata, a parasitic wasp of the Caribbean fruit fly, Anastrepha suspensa and other tephritid fruit flies. The DlEPV virion is 250–300 nm in diameter, has a “bumpy” appearance and a unipartite double stranded DNA genome of 290–300 kb. DlEPV DNA restriction fragment profiles differed from those reported for Amsacta moorei EPV (AmEPV) and Melanoplus sanguinipes EPV (MsEPV), the only two EPVs whose genomes have been sequenced, and from those reported for vaccinia (Vac), a vertebrate poxvirus (chordopoxvirus, ChPV). Blast search and ClustalW alignment of the amino acids deduced from the 2316 nucleotides of a DlEPV DNA fragment cloned from an EcoR1 genomic library revealed 75–78% homology with the putative DNA-directed RNA polymerases of AmEPV, MsEPV, and two ChPV homologs of the Vac J6R gene. Of the deduced 772 amino acids in the DlEPV sequence, 28.4% are conserved/substituted among the four poxviruses aligned, 12.9% occur in at least one EPV, 6.5% in at least one ChPV, 3.1% in at least one EPV and one ChPV, and 49.1% occur only in DlEPV. Although the RI-36-1 fragment represents a portion of the gene, it contains nucleotides that encode the NADFDGDE consensus sequence of known DNA-directed RNA polymerases. Western blots using a mouse polyclonal anti-DlEPV serum recognized six major protein bands in combined fractions of sucrose-purified DlEPV, at least one band in homogenates of male and female wasps, and at least two bands in host hemolymph that contained DlEPV virions. A digoxigenin-labeled DlEPV genomic DNA probe recognized DNA in dot-blots of male and female wasps. These results confirm that DlEPV is a true EPV and probably a member of the Group C EPVs. Unlike other EPVs, DlEPV does not express the spheroidin protein. Since it also replicates in both the wasp and fly, members of two different insect Orders, DlEPV may represent a new EPV Group, or a subgroup of the Group C viruses.
Starting Page	10
File Format	PDF
ISSN	15362442
e-ISSN	15362442
Journal	Journal of Insect Science
Volume Number	2
Language	English
Publisher	University of Arizona Library
Publisher Date	2002-05-28
Access Restriction	Open
Rights Holder	University of Arizona Library
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Insect Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in