NDLI: Selectivity and self-assembly in the control of a bacterial toxin by an antitoxic noncoding RNA pseudoknot

Content Provider	PubMed Central
Author	Short, Francesca L. Pei, Xue Y. Blower, Tim R. Ong, Shue-li Fineran, Peter C. Luisi, Ben F. Salmond, George P. C.
Abstract	Bacterial small RNAs perform numerous regulatory roles, including acting as antitoxic components in toxin–antitoxin systems. In type III toxin–antitoxin systems, small processed RNAs directly antagonize their toxin protein partners, and in the systems characterized the toxin and antitoxin components together form a trimeric assembly. In the present study, we sought to define how the RNA antitoxin, ToxI, inhibits its potentially lethal protein partner, ToxN. We show through cross-inhibition experiments with the ToxIN systems from Pectobacterium atrosepticum (ToxINPa) and Bacillus thuringiensis (ToxINBt) that ToxI RNAs are highly selective enzyme inhibitors. Both systems have an “addictive” plasmid maintenance phenotype. We demonstrate that ToxIPa can inhibit ToxNPa in vitro both in its processed form and as a repetitive precursor RNA, and this inhibition is linked to the self-assembly of the trimeric complex. Inhibition and self-assembly are both mediated entirely by the ToxIPa RNA, with no requirement for cellular factors or exogenous energy. Finally, we explain the origins of ToxI antitoxin selectivity through our crystal structure of the ToxINBt complex. Our results show how a processed RNA pseudoknot can inhibit a deleterious protein with exquisite molecular specificity and how these self-contained and addictive RNA-protein pairs can confer different adaptive benefits in their bacterial hosts.
Related Links	http://dx.doi.org/10.1073/pnas.1216039110
Starting Page	241
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	110
Language	English
Publisher	National Academy of Sciences
Publisher Date	2013-01-15
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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