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| Content Provider | PubMed Central |
|---|---|
| Author | Sun, Hui Xia, Yang Paudel, Omkar Yang, Xiao-ru Sham, James S. K. |
| Copyright Year | 2012 |
| Abstract | Chronic hypoxic pulmonary hypertension (CHPH) is associated with altered expression and function of cation channels in pulmonary arterial smooth muscle cells (PASMCs), but little is known for anion channels. The Ca2+-activated Cl− channel (CaCC), recently identified as TMEM16A, plays important roles in pulmonary vascular function. The present study sought to determine the effects of chronic hypoxia (CH) on the expression and function of CaCCs in PASMCs, and their contributions to the vascular hyperreactivity in CHPH. Male Wistar rats were exposed to room air or 10% O2 for 3–4 weeks to generate CHPH. CaCC current (I Cl.Ca) elicited by caffeine-induced Ca2+ release or by depolarization at a constant high [Ca2+]i (500 or 750 nm) was significantly larger in PASMCs of CH rats compared to controls. The enhanced I Cl.Ca density in CH PASMCs was unrelated to changes in amplitude of Ca2+ release, Ca2+-dependent activation, voltage-dependent properties or calcineurin-dependent modulation of CaCCs, but was associated with increased TMEM16A mRNA and protein expression. Maximal contraction induced by serotonin, an important mediator of CHPH, was potentiated in endothelium-denuded pulmonary arteries of CH rats. The enhanced contractile response was prevented by the CaCC blockers niflumic acid and T16Ainh-A01, or by the L-type Ca2+ channel antagonist nifedipine. The effects of niflumic acid and nifedipine were non-additive. Our results demonstrate for the first time that CH increases I Cl.Ca density, which is attributable to an upregulation of TMEM16A expression in PASMCs. The augmented CaCC activity in PASMCs may potentiate membrane depolarization and L-type channel activation in response to vasoconstrictors and enhance pulmonary vasoreactivity in CHPH. |
| Related Links | http://dx.doi.org/10.1113/jphysiol.2012.232520 |
| Ending Page | 3521 |
| Page Count | 15 |
| Starting Page | 3507 |
| File Format | |
| ISSN | 00223751 |
| e-ISSN | 14697793 |
| Journal | The Journal of Physiology |
| Issue Number | Pt 15 |
| Volume Number | 590 |
| Language | English |
| Publisher | Blackwell Science Inc |
| Publisher Date | 2012-08-01 |
| Access Restriction | Open |
| Rights Holder | Blackwell Science Inc |
| Subject Keyword | Physiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sports Science |
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