NDLI: Modulation of Ca2+-dependent Cl− channels by calcineurin in rabbit coronary arterial myocytes

Content Provider	PubMed Central
Author	Ledoux, Jonathan Greenwood, Iain Villeneuve, Louis R. Leblanc, Normand
Copyright Year	2003
Abstract	The role of the Ca2+-dependent phosphatase calcineurin (CaN) in the modulation of Ca2+-dependent Cl- channels (ClCa) was studied in freshly isolated rabbit coronary arterial myocytes. Immunocytochemical experiments showed that calmodulin-dependent protein kinase II (CaMKII) and CaN were distributed evenly throughout the cytoplasm of coronary myocytes at rest and translocated to the plasmalemma when intracellular Ca2+ was increased. ClCa currents (I Cl(Ca)) elicited by cell dialysis with fixed intracellular Ca2+ levels up to 500 nm were inhibited by 10 μ m cyclosporin A (CsA), a specific inhibitor of CaN, in a voltage-dependent manner, whereas currents evoked by 1 μ m Ca2+ were not affected. Inhibition of CaN with CsA also led to a significant reduction in Ca2+ sensitivity of the channel at +50 mV; half-maximal activation increased from 363 ± 16 nm Ca2+ in control to 515 ± 40 nm Ca2+ in the presence of CsA. Similar effects were observed on I Cl(Ca) when a specific peptide fragment inhibitor of CaN (CaN-AF, 5 μ m) was dialysed into the cell via the pipette (500 nm Ca2+). Application of KN-93 (10 μ m), a specific inhibitor of CaMKII, enhanced I Cl(Ca) in myocytes dialysed with 1 μ m Ca2+ but produced no significant effect on this current when the cells were dialysed with 350 or 500 nm Ca2+. These results are consistent with the notion that in coronary arterial cells, the activity of ClCa is enhanced by dephosphorylation of the channel or a closely associated regulatory protein. Moreover the balance of CaN and CaMKII regulating I Cl(Ca) is dependent on the level of Ca2+ used to activate I Cl(Ca).
Related Links	http://dx.doi.org/10.1113/jphysiol.2003.043836
Ending Page	714
Page Count	14
Starting Page	701
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 3
Volume Number	552
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2003-11-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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