NDLI: Mapping the Complex Morphology of Cell Interactions with Nanowire Substrates Using FIB-SEM

Content Provider	PubMed Central
Author	Wierzbicki, Rafał Carsten, Købler Jensen, Mikkel R. B. Łopacińska, Joanna Schmidt, Michael S. Skolimowski, Maciej Abeille, Fabien Qvortrup, Klaus Kristian, Mølhave
Editor	Rozhkova, Elena A.
Copyright Year	2013
Abstract	Using high resolution focused ion beam scanning electron microscopy (FIB-SEM) we study the details of cell-nanostructure interactions using serial block face imaging. 3T3 Fibroblast cellular monolayers are cultured on flat glass as a control surface and on two types of nanostructured scaffold substrates made from silicon black (Nanograss) with low- and high nanowire density. After culturing for 72 hours the cells were fixed, heavy metal stained, embedded in resin, and processed with FIB-SEM block face imaging without removing the substrate. The sample preparation procedure, image acquisition and image post-processing were specifically optimised for cellular monolayers cultured on nanostructured substrates. Cells display a wide range of interactions with the nanostructures depending on the surface morphology, but also greatly varying from one cell to another on the same substrate, illustrating a wide phenotypic variability. Depending on the substrate and cell, we observe that cells could for instance: break the nanowires and engulf them, flatten the nanowires or simply reside on top of them. Given the complexity of interactions, we have categorised our observations and created an overview map. The results demonstrate that detailed nanoscale resolution images are required to begin understanding the wide variety of individual cells’ interactions with a structured substrate. The map will provide a framework for light microscopy studies of such interactions indicating what modes of interactions must be considered.
Related Links	http://dx.doi.org/10.1371/journal.pone.0053307
Starting Page	53307
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	1
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-01-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Seeing a Mycobacterium-Infected Cell in Nanoscale 3D: Correlative Imaging by Light Microscopy and FIB/SEM Tomography

Three-Dimensional Numerical Model of Cell Morphology during Migration in Multi-Signaling Substrates

Functional Mapping of Protein-Protein Interactions in an Enzyme Complex by Directed Evolution

Localising Loci underlying Complex Trait Variation Using Regional Genomic Relationship Mapping

Differential Effects of Tissue Culture Coating Substrates on Prostate Cancer Cell Adherence, Morphology and Behavior

High Resolution Mapping of Enhancer-Promoter Interactions

Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)

Identification of Anaphase Promoting Complex Substrates in S. cerevisiae

Synthetic Morphology Using Alternative Inputs

Mapping the Complex Morphology of Cell Interactions with Nanowire Substrates Using FIB-SEM

Similar Documents

Seeing a Mycobacterium-Infected Cell in Nanoscale 3D: Correlative Imaging by Light Microscopy and FIB/SEM Tomography

Three-Dimensional Numerical Model of Cell Morphology during Migration in Multi-Signaling Substrates

Functional Mapping of Protein-Protein Interactions in an Enzyme Complex by Directed Evolution

Localising Loci underlying Complex Trait Variation Using Regional Genomic Relationship Mapping

Differential Effects of Tissue Culture Coating Substrates on Prostate Cancer Cell Adherence, Morphology and Behavior

High Resolution Mapping of Enhancer-Promoter Interactions

Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)

Identification of Anaphase Promoting Complex Substrates in S. cerevisiae

Synthetic Morphology Using Alternative Inputs

Mapping the Complex Morphology of Cell Interactions with Nanowire Substrates Using FIB-SEM