NDLI: Synergistic Action of Gentamicin and Bacteriophage in a Continuous Culture Population of Staphylococcus aureus

Content Provider	PubMed Central
Author	Kirby, Amy E.
Editor	Webber, Mark Alexander
Copyright Year	2012
Abstract	With the increasing frequency of antibiotic resistance and the decreasing frequency of new antibiotics entering the market, interest has returned to developing bacteriophage as a therapeutic agent. Acceptance of phage therapy, however, is limited by the unknown pharmacodynamics of a replicating agent, as well as the potential for the evolution of resistant bacteria. One way to overcome some of these limitations is to incorporate phage and antibiotics into a dual therapy regimen; however, this increases the complexity of the pharmacodynamics. The aim of this study is to develop an experimental system to evaluate the pharmacodynamics of dual phage-drug therapy. A continuous culture system for Staphylococcus aureus is used to simulate the pharmacokinetics of periodic antibiotic dosing alone and in combination with lytic phage. A computer model representation of the system allows further evaluation of the conditions governing the observed pharmacodynamics. The results of this experimental/modeling approach suggest that dual therapy can be more efficacious than single therapies, particularly if there is an overlap in the physiological pathways targeted by the individual agents. In this case, treatment with gentamicin induces a population of cells with a strong aggregation phenotype. These aggregators also have an increased ability to form biofilm, which is a well-known, non-genetic mechanism of drug resistance. However, the aggregators are also more susceptible than the parental strain to the action of the phage. Thus, dual treatment with gentamicin and phage resulted in lower final cell densities than either treatment alone. Unlike in the phage-only treatment, phage-resistant isolates were not detected in the dual treatment.
Related Links	http://dx.doi.org/10.1371/journal.pone.0051017
Starting Page	51017
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	11
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-11-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mobilization of Genomic Islands of Staphylococcus aureus by Temperate Bacteriophage

Microbiological and Molecular Assessment of Bacteriophage ISP for the Control of Staphylococcus aureus

The Influence of Staphylococcus aureus on Gut Microbial Ecology in an In Vitro Continuous Culture Human Colonic Model System

Population Structure of Staphylococcus aureus from Trinidad & Tobago

Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

Biofilms of a Bacillus subtilis Hospital Isolate Protect Staphylococcus aureus from Biocide Action

Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant

Population Diversification in Staphylococcus aureus Biofilms May Promote Dissemination and Persistence

Obesity and Staphylococcus aureus Nasal Colonization among Women and Men in a General Population

Synergistic Action of Gentamicin and Bacteriophage in a Continuous Culture Population of Staphylococcus aureus

Similar Documents

Mobilization of Genomic Islands of Staphylococcus aureus by Temperate Bacteriophage

Microbiological and Molecular Assessment of Bacteriophage ISP for the Control of Staphylococcus aureus

The Influence of Staphylococcus aureus on Gut Microbial Ecology in an In Vitro Continuous Culture Human Colonic Model System

Population Structure of Staphylococcus aureus from Trinidad & Tobago

Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

Biofilms of a Bacillus subtilis Hospital Isolate Protect Staphylococcus aureus from Biocide Action

Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant

Population Diversification in Staphylococcus aureus Biofilms May Promote Dissemination and Persistence

Obesity and Staphylococcus aureus Nasal Colonization among Women and Men in a General Population

Synergistic Action of Gentamicin and Bacteriophage in a Continuous Culture Population of Staphylococcus aureus