NDLI: Two Origins of Blastemal Progenitors Define Blastemal Regeneration of Zebrafish Lower Jaw

Content Provider	PubMed Central
Author	Wang, Xuelong He, Huihui Tang, Wenqiao Zhang, Xin A. Hua, Xianxin Yan, Jizhou
Editor	Pesce, Maurizio
Copyright Year	2012
Abstract	Zebrafish possess a remarkable ability to regenerate complicated structures by formation of a mass of undifferentiated mesenchymal cells called blastema. To understand how the blastema retains the original structural form, we investigate cellular transitions and transcriptional characteristics of cell identity genes during all stages of regeneration of an amputated lower jaw. We find that mesenchymal blastema originates from multiple sources including nucleated blood cells, fibroblasts, damaged muscle cells and pigment cells. These cells are transformed into two populations of blastemal progenitors: foxi1-expression and isl1-expression, before giving rise to cartilage, bone, and muscle. Time point- based transcriptomal analysis of 45 annotated Hox genes reveal that five 3′-end Hox genes and an equal number of 5′-end Hox genes are activated largely at the stage of blastema reformation. RNA in situ hybridization shows that foxi1 and pax3a are respectively expressed in the presumptive mandible skeletal region and regenerating muscle at 5 dpa. In contrast, hoxa2b and hoxa11b are widely expressed with different domain in chondrogenic blastema and blastema mesenchyme. Knockdown foxi1 changes the expression patterns of sox9a and hoxa2b in chondrogenic blastema. From these results we propose that two origins of blastemal progenitors define blastema skeleton and muscle respecifications through distinct signaling pathways. Meanwhile, the positional identity of blastema reformation is implicated in mesenchymal segmentation and characteristic expression pattern of Hox genes.
Related Links	http://dx.doi.org/10.1371/journal.pone.0045380
Starting Page	45380
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-09-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

csrnp1a Is Necessary for the Development of Primitive Hematopoiesis Progenitors in Zebrafish

Hypoxia/Reoxygenation Cardiac Injury and Regeneration in Zebrafish Adult Heart

Fgf and Sdf-1 Pathways Interact during Zebrafish Fin Regeneration

Augmenter of Liver Regeneration (alr) Promotes Liver Outgrowth during Zebrafish Hepatogenesis

In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration

Molecular and Cellular Characterization of a Zebrafish Optic Pathway Tumor Line Implicates Glia-Derived Progenitors in Tumorigenesis

Time point-based integrative analyses of deep-transcriptome identify four signal pathways in blastemal regeneration of zebrafish lower jaw.

Proliferative Regeneration of Zebrafish Lateral Line Hair Cells after Different Ototoxic Insults

V-ATPase Proton Pumping Activity Is Required for Adult Zebrafish Appendage Regeneration

Two Origins of Blastemal Progenitors Define Blastemal Regeneration of Zebrafish Lower Jaw

Similar Documents

csrnp1a Is Necessary for the Development of Primitive Hematopoiesis Progenitors in Zebrafish

Hypoxia/Reoxygenation Cardiac Injury and Regeneration in Zebrafish Adult Heart

Fgf and Sdf-1 Pathways Interact during Zebrafish Fin Regeneration

Augmenter of Liver Regeneration (alr) Promotes Liver Outgrowth during Zebrafish Hepatogenesis

In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration

Molecular and Cellular Characterization of a Zebrafish Optic Pathway Tumor Line Implicates Glia-Derived Progenitors in Tumorigenesis

Time point-based integrative analyses of deep-transcriptome identify four signal pathways in blastemal regeneration of zebrafish lower jaw.

Proliferative Regeneration of Zebrafish Lateral Line Hair Cells after Different Ototoxic Insults

V-ATPase Proton Pumping Activity Is Required for Adult Zebrafish Appendage Regeneration

Two Origins of Blastemal Progenitors Define Blastemal Regeneration of Zebrafish Lower Jaw