NDLI: High-Capacity Conductive Nanocellulose Paper Sheets for Electrochemically Controlled Extraction of DNA Oligomers

Content Provider	PubMed Central
Author	Razaq, Aamir Nyström, Gustav Strømme, Maria Albert, Mihranyan Nyholm, Leif
Editor	Lebedev, Nikolai
Copyright Year	2011
Abstract	Highly porous polypyrrole (PPy)-nanocellulose paper sheets have been evaluated as inexpensive and disposable electrochemically controlled three-dimensional solid phase extraction materials. The composites, which had a total anion exchange capacity of about 1.1 mol kg−1, were used for extraction and subsequent release of negatively charged fluorophore tagged DNA oligomers via galvanostatic oxidation and reduction of a 30–50 nm conformal PPy layer on the cellulose substrate. The ion exchange capacity, which was, at least, two orders of magnitude higher than those previously reached in electrochemically controlled extraction, originated from the high surface area (i.e. 80 m2 g−1) of the porous composites and the thin PPy layer which ensured excellent access to the ion exchange material. This enabled the extractions to be carried out faster and with better control of the PPy charge than with previously employed approaches. Experiments in equimolar mixtures of (dT)6, (dT)20, and (dT)40 DNA oligomers showed that all oligomers could be extracted, and that the smallest oligomer was preferentially released with an efficiency of up to 40% during the reduction of the PPy layer. These results indicate that the present material is very promising for the development of inexpensive and efficient electrochemically controlled ion-exchange membranes for batch-wise extraction of biomolecules.
Related Links	http://dx.doi.org/10.1371/journal.pone.0029243
Starting Page	29243
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	12
Volume Number	6
Language	English
Publisher	Public Library of Science
Publisher Date	2011-12-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Extraction of High Quality DNA from Seized Moroccan Cannabis Resin (Hashish)

Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays

DNA Extraction Columns Contaminated with Murine Sequences

An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing

DNA Extraction from Museum Specimens of Parasitic Hymenoptera

Low Cost Extraction and Isothermal Amplification of DNA for Infectious Diarrhea Diagnosis

The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues

Evaluating DNA Extraction Methods for Community Profiling of Pig Hindgut Microbial Community

Formation of High-Order Oligomers by a Hyperthemostable Fe-Superoxide Dismutase (tcSOD)

High-Capacity Conductive Nanocellulose Paper Sheets for Electrochemically Controlled Extraction of DNA Oligomers

Similar Documents

Extraction of High Quality DNA from Seized Moroccan Cannabis Resin (Hashish)

Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays

DNA Extraction Columns Contaminated with Murine Sequences

An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing

DNA Extraction from Museum Specimens of Parasitic Hymenoptera

Low Cost Extraction and Isothermal Amplification of DNA for Infectious Diarrhea Diagnosis

The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues

Evaluating DNA Extraction Methods for Community Profiling of Pig Hindgut Microbial Community

Formation of High-Order Oligomers by a Hyperthemostable Fe-Superoxide Dismutase (tcSOD)

High-Capacity Conductive Nanocellulose Paper Sheets for Electrochemically Controlled Extraction of DNA Oligomers