NDLI: Yeast pheromone receptor genes STE2 and STE3 are differently regulated at the transcription and polyadenylation level

Content Provider	PubMed Central
Author	Segni, Gianfranco Di Gastaldi, Serena Zamboni, Michela Tocchini-valentini, Glauco P.
Abstract	The orderly expression of specific genes is the basis for cell differentiation. Saccharomyces cerevisiae has two haploid mating types, a and α cells, in which the mating-specific genes are differentially expressed. When a and α cells are committed to mate, their growth is arrested. Here we show that a cryptic polyadenylation site is present inside the coding region of the a-specific STE2 gene, encoding the receptor for the α-factor. The two cell types produce an incomplete STE2 transcript, but only a cells generate full-length STE2 mRNA. We eliminated the cryptic poly(A) signal, thereby allowing the production of a complete STE2 mRNA in α cells. We mutagenized α cells and isolated a mutant producing full-length STE2 mRNA. The mutation occurred in the ITC1 gene, whose product, together with the product of ISW2, is known to repress STE2 transcriptional initiation. We propose that the regulation of the yeast mating genes is achieved through a concerted mechanism involving transcriptional and posttranscriptional events. In particular, the early poly(A) site in STE2 could contribute to a complete shutoff of its expression in α cells, avoiding autocrine activation and growth arrest. Remarkably, no cryptic poly(A) sites are present in the a-factor receptor STE3 gene, indicating that S. cerevisiae has devised different strategies to regulate the two receptor genes. It is predictable that a correlation between the repression of a gene and the presence of a cryptic poly(A) site could also be found in other organisms, especially when expression of that gene may be harmful.
Related Links	http://dx.doi.org/10.1073/pnas.1114648108
Ending Page	17086
Page Count	5
Starting Page	17082
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	41
Volume Number	108
Language	English
Publisher	National Academy of Sciences
Publisher Date	2011-10-11
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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