NDLI: Phosphorylation of the Yeast γ-Tubulin Tub4 Regulates Microtubule Function

Content Provider	PubMed Central
Author	Lin, Tien-chen Gombos, Linda Neuner, Annett Sebastian, Dominik Olsen, Jesper V. Hrle, Ajla Benda, Christian Schiebel, Elmar
Editor	Lustig, Arthur J.
Abstract	The yeast γ-tubulin Tub4 is assembled with Spc97 and Spc98 into the small Tub4 complex. The Tub4 complex binds via the receptor proteins Spc72 and Spc110 to the spindle pole body (SPB), the functional equivalent of the mammalian centrosome, where the Tub4 complex organizes cytoplasmic and nuclear microtubules. Little is known about the regulation of the Tub4 complex. Here, we isolated the Tub4 complex with the bound receptors from yeast cells. Analysis of the purified Tub4 complex by mass spectrometry identified more than 50 phosphorylation sites in Spc72, Spc97, Spc98, Spc110 and Tub4. To examine the functional relevance of the phosphorylation sites, phospho-mimicking and non-phosphorylatable mutations in Tub4, Spc97 and Spc98 were analyzed. Three phosphorylation sites in Tub4 were found to be critical for Tub4 stability and microtubule organization. One of the sites is highly conserved in γ-tubulins from yeast to human.
Related Links	http://dx.doi.org/10.1371/journal.pone.0019700
Starting Page	19700
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	5
Volume Number	6
Language	English
Publisher	Public Library of Science
Publisher Date	2011-05-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Phosphorylation of the Yeast γ-Tubulin Tub4 Regulates Microtubule Function

The Role of γ-Tubulin in Centrosomal Microtubule Organization

Correction: The Role of γ-Tubulin in Centrosomal Microtubule Organization

Phosphorylation Regulates SIRT1 Function

Mutation of Ser172 in Yeast β Tubulin Induces Defects in Microtubule Dynamics and Cell Division

Virtual and Biophysical Screening Targeting the γ-Tubulin Complex – A New Target for the Inhibition of Microtubule Nucleation

Cell-cycle dependent phosphorylation of yeast pericentrin regulates γ-TuSC-mediated microtubule nucleation

Fission Yeast Mto1 Regulates Diversity of Cytoplasmic Microtubule Organizing Centers

EB1 Recognizes the Nucleotide State of Tubulin in the Microtubule Lattice

Phosphorylation of the Yeast γ-Tubulin Tub4 Regulates Microtubule Function

Similar Documents

Phosphorylation of the Yeast γ-Tubulin Tub4 Regulates Microtubule Function

The Role of γ-Tubulin in Centrosomal Microtubule Organization

Correction: The Role of γ-Tubulin in Centrosomal Microtubule Organization

Phosphorylation Regulates SIRT1 Function

Mutation of Ser172 in Yeast β Tubulin Induces Defects in Microtubule Dynamics and Cell Division

Virtual and Biophysical Screening Targeting the γ-Tubulin Complex – A New Target for the Inhibition of Microtubule Nucleation

Cell-cycle dependent phosphorylation of yeast pericentrin regulates γ-TuSC-mediated microtubule nucleation

Fission Yeast Mto1 Regulates Diversity of Cytoplasmic Microtubule Organizing Centers

EB1 Recognizes the Nucleotide State of Tubulin in the Microtubule Lattice

Phosphorylation of the Yeast γ-Tubulin Tub4 Regulates Microtubule Function