NDLI: Genomic Characterization and High Prevalence of Bocaviruses in Swine

Content Provider	PubMed Central
Author	Shan, Tongling Lan, Daoliang Li, Linlin Wang, Chunmei Cui, Li Zhang, Wen Hua, Xiuguo Zhu, Caixia Zhao, Wei Delwart, Eric
Editor	Bausch, Daniel G.
Abstract	Using random PCR amplification followed by plasmid subcloning and DNA sequencing, we detected bocavirus related sequences in 9 out of 17 porcine stool samples. Using primer walking, we sequenced the nearly complete genomes of two highly divergent bocaviruses we provisionally named porcine bocavirus 1 isolate H18 (PBoV1-H18) and porcine bocavirus 2 isolate A6 (PBoV2-A6) which differed by 51.8% in their NS1 protein. Phylogenetic analysis indicated that PBoV1-H18 was very closely related to a ∼2 Kb central region of a porcine bocavirus-like virus (PBo-LikeV) from Sweden described in 2009. PBoV2-A6 was very closely related to the porcine bocavirus genomes PBoV-1 and PBoV2 from China described in 2010. Among 340 fecal samples collected from different age, asymptomatic swine in five Chinese provinces, the prevalence of PBoV1-H18 and PBoV2-A6 related viruses were 45–75% and 55–70% respectively, with 30–47% of pigs co-infected. PBoV1-A6 related strains were highly conserved, while PBoV2-H18 related strains were more diverse, grouping into two genotypes corresponding to the previously described PBoV1 and PBoV2. Together with the recently described partial bocavirus genomes labeled V6 and V7, a total of three major porcine bocavirus clades have therefore been described to date. Further studies will be required to elucidate the possible pathogenic impact of these diverse bocaviruses either alone or in combination with other porcine viruses.
Related Links	http://dx.doi.org/10.1371/journal.pone.0017292
Starting Page	17292
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	4
Volume Number	6
Language	English
Publisher	Public Library of Science
Publisher Date	2011-04-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Genomic Characterization and High Prevalence of Bocaviruses in Swine

Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses

Seroepidemiology of Human Bocaviruses 1 and 2 in China

Genomic Characterization of the Taylorella Genus

Prevalence of Hepatitis E Virus in Swine Fed on Kitchen Residue

Genomic Characterization of Campylobacter jejuni Strain M1

Rapid Genomic Characterization of the Genus Vitis

Genomic Characterization of Methanomicrobiales Reveals Three Classes of Methanogens

High-Throughput Cryopreservation of In Vivo-Derived Swine Embryos

Genomic Characterization and High Prevalence of Bocaviruses in Swine

Similar Documents

Genomic Characterization and High Prevalence of Bocaviruses in Swine

Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses

Seroepidemiology of Human Bocaviruses 1 and 2 in China

Genomic Characterization of the Taylorella Genus

Prevalence of Hepatitis E Virus in Swine Fed on Kitchen Residue

Genomic Characterization of Campylobacter jejuni Strain M1

Rapid Genomic Characterization of the Genus Vitis

Genomic Characterization of Methanomicrobiales Reveals Three Classes of Methanogens

High-Throughput Cryopreservation of In Vivo-Derived Swine Embryos

Genomic Characterization and High Prevalence of Bocaviruses in Swine