NDLI: Spatially Directed Guidance of Stem Cell Population Migration by Immobilized Patterns of Growth Factors

Content Provider	PubMed Central
Author	Miller, Eric D. Li, Kang Kanade, Takeo Weiss, Lee E. Walker, Lynn M. Campbell, Phil G.
Abstract	We investigated how engineered gradients of exogenous growth factors, immobilized to an extracellular matrix material, influence collective guidance of stem cell populations over extended time (>1 day) and length (>1 mm) scales in vitro. Patterns of low-to-high, high-to-low, and uniform concentrations of heparin-binding epidermal growth factor-like growth factor were inkjet printed at precise locations on fibrin substrates. Proliferation and migration responses of mesenchymal stem cells seeded at pattern origins were observed with time-lapse video microscopy and analyzed using both manual and automated computer vision-based cell tracking techniques. Based on results of established chemotaxis studies, we expected that the low-to-high gradient would most effectively direct cell guidance away from the cell source. All printed patterns, however, were found to direct net collective cell guidance with comparable responses. Our analysis revealed that collective “cell diffusion” down a cell-to-cell confinement gradient originating at the cell starting lines and not the net sum of directed individual cell migration up a growth factor concentration gradient is the principal driving force for directing mesenchymal stem cell population outgrowth from a cell source. These results suggest that simple uniform distributions of growth factors immobilized to an extracellular matrix material may be as effective in directing cell migration into a wound site as more complex patterns with concentration gradients.
Related Links	http://dx.doi.org/10.1016/j.biomaterials.2010.12.005
Ending Page	2785
Page Count	11
Starting Page	2775
File Format	PDF
ISSN	01429612
e-ISSN	18785905
Journal	Biomaterials
Issue Number	11
Volume Number	32
Language	English
Publisher Date	2011-04-01
Access Restriction	Open
Subject Keyword	Biophysics Mechanics of Materials Bioengineering Biomaterials Ceramics and Composites Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Nanoscience and Nanotechnology Mechanics of Materials Biomaterials Biophysics Bioengineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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