NDLI: Directed Endothelial Cell Morphogenesis in Micropatterned Gelatin Methacrylate Hydrogels

Content Provider	PubMed Central
Author	Mehdi, Nikkhah Eshak, Nouran Zorlutuna, Pinar Annabi, Nasim Castello, Marco Kim, Keekyoung Dolatshahi-pirouz, Alireza Edalat, Faramarz Bae, Hojae Yang, Yunzhi Khademhosseini, Ali
Copyright Year	2012
Abstract	Engineering of organized vasculature is a crucial step in the development of functional and clinically relevant tissue constructs. A number of previous techniques have been proposed to spatially regulate the distribution of angiogenic biomolecules and vascular cells within biomaterial matrices to promote vascularization. Most of these approaches have been limited to two-dimensional (2D) micropatterned features or have resulted in formation of random vasculature within three-dimensional (3D) microenvironments. In this study, we investigate 3D endothelial cord formation within micropatterned gelatin methacrylate (GelMA) hydrogels with varying geometrical features (50–150 µm height). We demonstrated the significance dependence of endothelial cells proliferation, alignment and cord formation on geometrical dimensions of the patterned features. The cells were able to align and organize within the micropatterned constructs and assemble to form cord structures with organized actin fibers and circular/elliptical cross-sections. The inner layer of the cord structure was filled with gel showing that the micropatterned hydrogel constructs guided the assembly of endothelial cells into cord structures. Notably, the endothelial cords were retained within the hydrogel microconstructs for all geometries after two weeks of culture; however, only the 100 µm-high constructs provided the optimal microenvironment for the formation of circular and stable cord structures. Our findings suggest that endothelial cord formation is a preceding step to tubulogenesis and the proposed system can be used to develop organized vasculature for engineered tissue constructs.
Related Links	http://dx.doi.org/10.1016/j.biomaterials.2012.08.068
Ending Page	9018
Page Count	10
Starting Page	9009
File Format	PDF
ISSN	01429612
e-ISSN	18785905
Journal	Biomaterials
Issue Number	35
Volume Number	33
Language	English
Publisher Date	2012-12-01
Access Restriction	Open
Subject Keyword	Biophysics Mechanics of Materials Bioengineering Biomaterials Ceramics and Composites Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Nanoscience and Nanotechnology Mechanics of Materials Biomaterials Biophysics Bioengineering

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