NDLI: Cytoplasmic Domain of P-selectin Glycoprotein Ligand-1 Facilitates Dimerization and Export from the Endoplasmic Reticulum*

Content Provider	PubMed Central
Author	Miner, Jonathan J. Shao, Bojing Wang, Ying Chichili, Gurunadh R. Liu, Zhenghui Klopocki, Arkadiusz G. Yago, Tadayuki Mcdaniel, J. Michael Rodgers, William Xia, Lijun Mcever, Rodger P.
Abstract	P-selectin glycoprotein ligand-1 (PSGL-1) is a homodimeric transmembrane mucin on leukocytes. During inflammation, reversible interactions of PSGL-1 with selectins mediate leukocyte rolling on vascular surfaces. The transmembrane domain of PSGL-1 is required for dimerization, and the cytoplasmic domain propagates signals that activate β2 integrins to slow rolling on integrin ligands. Leukocytes from knock-in “ΔCD” mice express a truncated PSGL-1 that lacks the cytoplasmic domain. Unexpectedly, they have 10-fold less PSGL-1 on their surfaces than WT leukocytes. Using glycosidases, proteases, Western blotting, confocal microscopy, cell-surface cross-linking, FRET, and pulse-chase metabolic labeling, we demonstrate that deleting the cytoplasmic domain impaired dimerization and delayed export of PSGL-1 from the endoplasmic reticulum (ER), markedly increasing a monomeric precursor in the ER and decreasing mature PSGL-1 on the cell surface. A monomeric full-length PSGL-1 made by substituting the transmembrane domain with that of CD43 exited the ER normally, revealing that dimerization was not required for ER export. Thus, the transmembrane and cytoplasmic domains cooperate to promote dimerization of PSGL-1. Furthermore, the cytoplasmic domain provides a key signal to export precursors of PSGL-1 from the ER to the Golgi apparatus en route to the cell surface.
Related Links	http://dx.doi.org/10.1074/jbc.m110.208777
Ending Page	9586
Page Count	10
Starting Page	9577
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	11
Volume Number	286
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2011-03-18
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Cytoplasmic Domain of P-selectin Glycoprotein Ligand-1 Facilitates Dimerization and Export from the Endoplasmic Reticulum

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Cytoplasmic Domain of P-selectin Glycoprotein Ligand-1 Facilitates Dimerization and Export from the Endoplasmic Reticulum*

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