NDLI: Ligand dimensions are important in controlling NK-cell responses

Content Provider	PubMed Central
Author	Brzostek, Joanna Chai, Jian-guo Gebhardt, Friedemann Busch, Dirk H. Zhao, Rui Merwe, P. Anton Van Der Gould, Keith G.
Abstract	Size-dependent protein segregation at the cell–cell contact interface has been suggested to be critical for regulation of lymphocyte function. We investigated the role of ligand dimensions in regulation of mouse NK-cell activation and inhibition. Elongated forms of H60a, a mouse NKG2D ligand, were generated and expressed stably in the RMA cell line. RMA cells expressing the normal size H60a were lysed efficiently by both freshly isolated and IL-2 stimulated C57BL/6 mouse-derived NK cells; however the level of lysis decreased as the H60a ligand size increased. Importantly, H60a elongation did not affect NKG2D binding, as determined by soluble NKG2D tetramer staining, and by examining NK-cell target cell conjugate formation. CHO cells are efficient at activating NK cells from C57BL/6 mice, and expression of a single chain form of H-2Kb, a ligand for the mouse inhibitory receptor Ly49C, strongly inhibited such activation of Ly49C/I positive NK cells. Elongation of H-2Kb resulted in decreased inhibition of both lysis and IFN-γ production by NK cells. These results establish that small ligand dimensions are important for both NK-cell activation and inhibition, and suggest that there are shared features between the mechanisms of receptor triggering on different types of lymphocytes.
Related Links	http://dx.doi.org/10.1002/eji.201040335
Ending Page	2059
Page Count	10
Starting Page	2050
File Format	PDF
ISSN	00142980
e-ISSN	15214141
Journal	European Journal of Immunology
Issue Number	7
Volume Number	40
Language	English
Publisher	WILEY-VCH Verlag
Publisher Date	2010-07-01
Access Restriction	Open
Rights Holder	WILEY-VCH Verlag
Subject Keyword	Immunology Immunology and Allergy Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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