NDLI: Reduction of diffusion barriers in isolated rat islets improves survival, but not insulin secretion or transplantation outcome

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Reduction of diffusion barriers in isolated rat islets improves survival, but not insulin secretion or transplantation outcome

Content Provider	PubMed Central
Author	Williams, S. Janette Huang, Han-hung Kover, Karen Moore, Wayne Berkland, Cory Singh, Milind Macgregor, Ronal Lisa, Stehno-bittel Smirnova, Irina V.
Copyright Year	2010
Abstract	For people with type 1 diabetes and severe hypoglycemic unawareness, islet transplants offer hope for improving the quality of life. However, islet cell death occurs quickly during or after transplantation, requiring large quantities of islets per transplant. The purpose of this study was to determine whether poor function demonstrated in large islets was a result of diffusion barriers and if removing those barriers could improve function and transplantation outcomes. Islets were isolated from male DA rats and measured for cell viability, islet survival, glucose diffusion and insulin secretion. Modeling of diffusion barriers was completed using dynamic partial differential equations for a sphere. Core cell death occurred in 100% of the large islets (diameter >150 µm), resulting in poor survival within 7 days after isolation. In contrast, small islets (diameter <100 µm) exhibited good survival rates in culture (91%). Glucose diffusion into islets was tracked with 2-NBDG; 4.2 µm/min in small islets and 2.8 µm/min in large islets. 2-NBDG never permeated to the core cells of islets larger than 150 µm diameter. Reducing the diffusion barrier in large islets improved their immediate and long-term viability in culture. However, reduction of the diffusion barrier in large islets failed to improve their inferior in vitro insulin secretion compared to small islets, and did not return glucose control to diabetic animals following transplantation. Thus, diffusion barriers lead to low viability and poor survival for large islets, but are not solely responsible for the inferior insulin secretion or poor transplantation outcomes of large versus small islets.
Starting Page	115
File Format	PDF
ISSN	15558592
e-ISSN	15558592
Journal	Organogenesis
Issue Number	2
Volume Number	6
Language	English
Publisher	Landes Bioscience
Publisher Date	2010-01-01
Access Restriction	Open
Rights Holder	Landes Bioscience
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Transplantation Embryology Biomedical Engineering

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