NDLI: A hyperactive quantitative trait locus allele of Arabidopsis BRX contributes to natural variation in root growth vigor

Content Provider	PubMed Central
Author	Beuchat, Julien Li, Shuwei Ragni, Laura Shindo, Chikako Kohn, Michael H. Hardtke, Christian S.
Abstract	Quantitative trait loci analysis of natural Arabidopsis thaliana accessions is increasingly exploited for gene isolation. However, to date this has mostly revealed deleterious mutations. Among them, a loss-of-function allele identified the root growth regulator BREVIS RADIX (BRX). Here we present evidence that BRX and the paralogous BRX-LIKE (BRXL) genes are under selective constraint in monocotyledons as well as dicotyledons. Unexpectedly, however, whereas none of the Arabidopsis orthologs except AtBRXL1 could complement brx null mutants when expressed constitutively, nearly all monocotyledon BRXLs tested could. Thus, BRXL proteins seem to be more diversified in dicotyledons than in monocotyledons. This functional diversification was correlated with accelerated rates of sequence divergence in the N-terminal regions. Population genetic analyses of 30 haplotypes are suggestive of an adaptive role of AtBRX and AtBRXL1. In two accessions, Lc-0 and Lov-5, seven amino acids are deleted in the variable region between the highly conserved C-terminal, so-called BRX domains. Genotyping of 42 additional accessions also found this deletion in Kz-1, Pu2-7, and Ws-0. In segregating recombinant inbred lines, the Lc-0 allele (AtBRXLc-0) conferred significantly enhanced root growth. Moreover, when constitutively expressed in the same regulatory context, AtBRXLc-0 complemented brx mutants more efficiently than an allele without deletion. The same was observed for AtBRXL1, which compared with AtBRX carries a 13 amino acid deletion that encompasses the deletion found in AtBRXLc-0. Thus, the AtBRXLc-0 allele seems to contribute to natural variation in root growth vigor and provides a rare example of an experimentally confirmed, hyperactive allelic variant.
Related Links	http://dx.doi.org/10.1073/pnas.0913207107
Ending Page	8480
Page Count	6
Starting Page	8475
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	18
Volume Number	107
Language	English
Publisher	National Academy of Sciences
Publisher Date	2010-05-04
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

QTLs underlying natural variation of root growth angle among rice cultivars with the same functional allele of DEEPER ROOTING 1 springerOpen/

Natural genetic variation of root system architecture from Arabidopsis to Brachypodium: towards adaptive value

Root penetration index 3, a major quantitative trait locus (QTL) associated with root system penetrability in Arabidopsis. (Dataset)

Genetic Interaction between Arabidopsis Qpm3.1 Locus and Bacterial Effector Gene hopW1-1 Underlies Natural Variation in Quantitative Disease Resistance to Pseudomonas Infection

Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis

Evolutionary dynamics of an Arabidopsis insect resistance quantitative trait locus

RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis

Quantitative trait locus mapping and DNA array hybridization identify an FLM deletion as a cause for natural flowering-time variation.

Quantitative trait locus mapping and DNA array hybridization identify an FLM deletion as a cause for natural flowering-time variation

A hyperactive quantitative trait locus allele of Arabidopsis BRX contributes to natural variation in root growth vigor

Similar Documents

QTLs underlying natural variation of root growth angle among rice cultivars with the same functional allele of DEEPER ROOTING 1 springerOpen/

Natural genetic variation of root system architecture from Arabidopsis to Brachypodium: towards adaptive value

Root penetration index 3, a major quantitative trait locus (QTL) associated with root system penetrability in Arabidopsis. (Dataset)

Genetic Interaction between Arabidopsis Qpm3.1 Locus and Bacterial Effector Gene hopW1-1 Underlies Natural Variation in Quantitative Disease Resistance to Pseudomonas Infection

Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis

Evolutionary dynamics of an Arabidopsis insect resistance quantitative trait locus

RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis

Quantitative trait locus mapping and DNA array hybridization identify an FLM deletion as a cause for natural flowering-time variation.

Quantitative trait locus mapping and DNA array hybridization identify an FLM deletion as a cause for natural flowering-time variation

A hyperactive quantitative trait locus allele of Arabidopsis BRX contributes to natural variation in root growth vigor