NDLI: CRYSTALLIZATION OF SYNTHETIC HAEMOZOIN (β-HAEMATIN) NUCLEATED AT THE SURFACE OF LIPID PARTICLES

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CRYSTALLIZATION OF SYNTHETIC HAEMOZOIN (β-HAEMATIN) NUCLEATED AT THE SURFACE OF LIPID PARTICLES

Content Provider	PubMed Central
Author	Hoang, Anh N. Ncokazi, Kanyile K. Villiers, Katherine A. De Wright, David W. Egan, Timothy J.
Abstract	The mechanism of formation of haemozoin, a detoxification by-product of several blood-feeding organisms including malaria parasites, has been a subject of debate; however, recent studies suggest that neutral lipids may serve as a catalyst. In this study, a model system consisting of an emulsion of neutral lipid particles was employed to investigate the formation of β-haematin, the synthetic counterpart of haemozoin, at the lipid-water interface. A solution of monoglyceride, either monostearoylglycerol (MSG) or monopalmitoylglycerol (MPG), dissolved in acetone and methanol was introduced to an aqueous surface. Fluorescence, confocal and transmission electron microscopic (TEM) imaging and dynamic light scattering analysis of samples obtained from beneath the surface confirmed the presence of homogeneous lipid particles existing in two major populations: one in the low micrometer size range and the other in the hundred nanometre range. The introduction of haem (Fe(III)PPIX) to this lipid particle system under biomimetic conditions (37 °C, pH 4.8) produced β-haematin with apparent first order kinetics and an average half life of 0.5 min. TEM of monoglycerides (MSG or MPG) extruded through a 200 nm filter with haem produced β-haematin crystals aligned and parallel to the lipid/water interface. These TEM data, together with a model system replacing the lipid with an aqueous organic solvent interface using either methyl laurate or docosane demonstrated that the OH and C=O groups are apparently necessary for efficient nucleation. This suggests that β-haematin crystallizes via epitaxial nucleation at the lipid-water interface through interaction of Fe(III)PPIX with the polar head group. Once nucleated, the crystal grows parallel to the interface until growth is terminated by the curvature of the lipid particle. The hydrophobic nature of the mature crystal favours an interior transport resulting in crystals aligned parallel to the lipid-water interface and each other, strikingly similar to that seen in malaria parasites.
Related Links	http://dx.doi.org/10.1039/b914359a
Ending Page	1244
Page Count	10
Starting Page	1235
File Format	PDF
ISSN	14779226
e-ISSN	14779234
Journal	Dalton transactions (Cambridge, England : 2003)
Issue Number	5
Volume Number	39
Language	English
Publisher Date	2010-01-01
Access Restriction	Open
Subject Keyword	Inorganic Chemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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Crystallization of synthetic haemozoin (β-haematin) nucleated at the surface of lipid particles

Crystallization of synthetic haemozoin (beta-haematin) nucleated at the surface of lipid particles.

Neutral lipids associated with haemozoin mediate efficient and rapid β-haematin formation at physiological pH, temperature and ionic composition

Neutral lipids associated with haemozoin mediate efficient and rapid β-haematin formation at physiological pH, temperature and ionic composition

Kinetics of competitive crystallization of β- and α-modifications in β-nucleated iPP studied by isothermal stepwise crystallization technique

DSC and morphological studies on the crystallization behavior of β-nucleated isotactic polypropylene composites filled with Kevlar fibers

Effect of blending way on β-crystallization tendency of compatibilized β-nucleated isotactic polypropylene/poly(ethylene terephthalate) blends

Synthetic Hemozoin (β-Hematin) Crystals Nucleate at the Surface of Neutral Lipid Droplets that Control Their Sizes

Nonisothermal crystallization kinetics of isotactic polypropylene nucleated with a novel supported β-nucleating agent

CRYSTALLIZATION OF SYNTHETIC HAEMOZOIN (β-HAEMATIN) NUCLEATED AT THE SURFACE OF LIPID PARTICLES

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Crystallization of synthetic haemozoin (β-haematin) nucleated at the surface of lipid particles

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CRYSTALLIZATION OF SYNTHETIC HAEMOZOIN (β-HAEMATIN) NUCLEATED AT THE SURFACE OF LIPID PARTICLES