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Inhibition of eNOS/sGC/PKG Pathway Decreases Akt Phosphorylation Induced by Kainic Acid in Mouse Hippocampus

Content Provider	PubMed Central
Author	Lee, Sang Hyun Byun, Jong Seon Kong, Pil Jae Lee, Hee Jae Kim, Duk Kyung Kim, Hae Sung Sohn, Jong-hee Lee, Jae Jun Lim, So Young Chun, Wanjoo Kim, Sung Soo
Copyright Year	2010
Abstract	The serine/threonine kinase Akt has been shown to play a role of multiple cellular signaling pathways and act as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI3K). It has been reported that phosphorylated Akt activates eNOS resulting in the production of NO and that NO stimulates soluble guanylate cyclase (sGC), which results in accumulation of cGMP and subsequent activation of the protein kinase G (PKG). It has been also reported that PKG activates PI3K/Akt signaling. Therefore, it is possible that PI3K, Akt, eNOS, sGC, and PKG form a loop to exert enhanced and sustained activation of Akt. However, the existence of this loop in eNOS-expressing cells, such as endothelial cells or astrocytes, has not been reported. Thus, we examined a possibility that Akt phosphorylation might be enhanced via eNOS/sGC/PKG/PI3K pathway in astrocytes in vivo and in vitro. Phosphorylation of Akt was detected in astrocytes after KA treatment and was maintained up to 72 h in mouse hippocampus. 2 weeks after KA treatment, astrocytic Akt phosphorylation was normalized to control. The inhibition of eNOS, sGC, and PKG significantly decreased Akt and eNOS phosphorylation induced by KA in astrocytes. In contrast, the decreased phosphorylation of Akt and eNOS by eNOS inhibition was significantly reversed with PKG activation. The above findings in mouse hippocampus were also observed in primary astrocytes. These data suggest that Akt/eNOS/sGC/PKG/PI3K pathway may constitute a loop, resulting in enhanced and sustained Akt activation in astrocytes.
Related Links	http://dx.doi.org/10.4196/kjpp.2010.14.1.37
Starting Page	37
File Format	PDF
ISSN	12264512
Journal	The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Issue Number	1
Volume Number	14
Language	English
Publisher	The Korean Physiological Society and The Korean Society of Pharmacology
Publisher Date	2010-01-01
Access Restriction	Open
Rights Holder	The Korean Physiological Society and The Korean Society of Pharmacology
Subject Keyword	Physiology Pharmacology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Pharmacology

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