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| Content Provider | PubMed Central |
|---|---|
| Author | Kirschenlohr, H. L. Metcalfe, J. C. Morris, P. G. Rodrigo, G. C. Smith, G. A. |
| Abstract | 19F NMR indicators have been used to measure the free cytosolic cation concentrations ([Mn+]i, where M is the atomic symbol and n is the value of the charge) of Ca2+, H+, and Mg2+ in perfused ferret hearts. The [Ca2+]i transient, cytosolic pH (pHi), and [Mg2+]i have also been followed at 16 phases in the cardiac cycle in hearts paced at 1.25 Hz at 30 degrees C. The initial [Ca2+]i rose rapidly after a 50-ms delay, was maximal at greater than 1.5 microM after 150 ms, and declined thereafter to the initial concentration. In contrast, no significant changes in pHi (pH 7.03 +/- 0.08) or [Mg2+]i (1.2 +/- 0.1 mM) were detected in the cycle. A decrease in developed pressure when the [Ca2+]i indicator (but not the pHi or [Mg2+]i indicator) was loaded into hearts was substantially reversed by the addition of 50 microM ZnCl2 to the perfusion medium. The Zn2+ was taken up into the myoplasm and displaced Ca2+ bound to the indicator, a symmetrically substituted difluoro derivative of 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (5FBAPTA), as evidenced by the appearance of the Zn-5FBAPTA resonance. The decrease in developed pressure caused by 5FBAPTA, therefore, may be due to its Ca2+ buffering effect on the myoplasm. By coloading hearts with the [Ca2+]i and pHi indicators, simultaneous measurement of several [Mn+]i was demonstrated, which should provide a useful addition to the methods available to monitor cardiac function and pharmacology. |
| Starting Page | 9017 |
| File Format | |
| ISSN | 10916490 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 23 |
| Volume Number | 85 |
| Language | English |
| Publisher Date | 1988-12-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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