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| Content Provider | PubMed Central |
|---|---|
| Author | Domeier, Timothy L. Blatter, Lothar A. Zima, Aleksey V. |
| Abstract | Many physiological processes and pharmacological agents modulate the ryanodine receptor (RyR), the primary sarcoplasmic reticulum (SR) Ca2+ release channel in the heart. However, how such modulations translate into functional effects during cardiac excitation–contraction coupling (ECC) is much less clear. Using a low dose (250 μm) of caffeine we sensitized the RyR and examined SR Ca2+ release using dynamic measurements of cytosolic Ca2+ ([Ca2+]i) and free Ca2+ within the SR ([Ca2+]SR). In field stimulated (1 Hz) rabbit ventricular myocytes, application of 250 μm caffeine caused an initial 33% increase in SR Ca2+ release, which was followed by a decrease in SR Ca2+ load (28%) and steady-state SR Ca2+ release (23%). To investigate the effects of caffeine on local SR Ca2+ release, we measured [Ca2+]SR from individual release junctions during ECC as well as during spontaneous Ca2+ sparks. In intact myocytes during ECC, caffeine increased global fractional SR Ca2+ release by decreasing the [Ca2+]SR level at which local release terminated by 21%. Similarly, in permeabilized myocytes during spontaneous Ca2+ sparks, caffeine decreased the [Ca2+]SR level for release termination by 12%. Finally, we examined if Ca2+ release termination was changed in myocytes from failing hearts, where remodelling processes lead to altered RyR function. In myocytes from failing rabbit hearts, the [Ca2+]SR termination level for Ca2+ sparks was 13% lower than that of non-failing myocytes. Collectively, these data suggest that altering the termination level for local Ca2+ release may represent a novel mechanism to increase SR Ca2+ release and contractility during ECC. |
| Related Links | http://dx.doi.org/10.1113/jphysiol.2009.177576 |
| Ending Page | 5209 |
| Page Count | 13 |
| Starting Page | 5197 |
| File Format | |
| ISSN | 00223751 |
| e-ISSN | 14697793 |
| Journal | The Journal of Physiology |
| Issue Number | Pt 21 |
| Volume Number | 587 |
| Language | English |
| Publisher | Blackwell Science Inc |
| Publisher Date | 2009-11-01 |
| Access Restriction | Open |
| Rights Holder | Blackwell Science Inc |
| Subject Keyword | Physiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sports Science |
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