NDLI: Vesicular release mode shapes the postsynaptic response at hippocampal synapses

Content Provider	PubMed Central
Author	Richards, David A.
Abstract	Release of neurotransmitters from synaptic vesicles is a central event in synaptic transmission. Recent evidence suggests that synaptic vesicles fuse with the plasma membrane by multiple routes during exocytosis, but the regulation and physiological implications of this choice are unclear. At hippocampal synapses, two modes of synaptic vesicle exocytosis can be distinguished by virtue of the rate and extent of loss of a fluorescent lipid marker (FM1-43). Here these two modes of exocytosis were investigated with a combination of electrophysiological recording and fluorescence imaging. It is shown that these exocytic modes result in distinct postsynaptic consequences, such that so-called ‘kiss-and-run’ exocytosis results in negligible activation of AMPA receptors, compared to the robust postsynaptic responses elicited by apparent full fusion. In contrast NMDA receptors are robustly activated by this form of glutamate delivery. Addition of cyclothiazide, which blocks AMPA receptor desensitization, reveals that the relatively slow rate of release of glutamate during kiss-and-run exocytosis shifts the population of AMPA receptors into a desensitized state, rather than simply being insufficient for receptor activation. These findings provide further support for the existence of a fusion pore mediated mode of exocytosis, and demonstrate that these two exocytic modes directly affect the throughput of synaptic transmission.
Related Links	http://dx.doi.org/10.1113/jphysiol.2009.175315
Ending Page	5080
Page Count	8
Starting Page	5073
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 21
Volume Number	587
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2009-11-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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