NDLI: Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture

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Erratum

Year: 2007, Volume: 39

Year: 2002, Volume: 34

Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture

Content Provider	PubMed Central
Author	Park, Yong Bin Kim, Yoon Young Oh, Sun Kyung Chung, Sun Gan Ku, Seung-yup Kim, Seok Hyun Choi, Young Min Moon, Shin Yong
Copyright Year	2008
Abstract	Human embryonic stem cells (hESCs) are considered to be able to stably maintain their characteristics in vitro for prolonged periods, but we had previously encountered changes in proliferative ability and differentiation potential during extended culture of hESCs. Therefore, we investigated the proliferative ability and differentiation potential of hESCs during long-term culture. The hESCs, SNUhES3, were used to analyze population-doubling time, proliferation rate and differentiation potential. We classified hESCs into three groups according to culture period. Ten colonies of hESCs for each group were daily measured colony area and population-doubling time was assessed by the changes of colony area. Proliferation rate of hESCs was measured by 5-bromo-2'-deoxyuridine (BrdU) assay and telomerase activity. To evaluate differentiation potentials for hESCs, expression levels of undifferentiated and/or differentiated hESCs markers were examined by FACS, RT-PCR and immunostaining. Population-doubling time of early passage hESCs was longer than those of middle or late passage. Proliferative ability of hESCs was accelerated depending on culture periods. Cellular morphologies and the expression level of each three germ layer markers were obviously different from each passage of reattached embryoid bodies (EBs) after spontaneous differentiation. Differentiated cells of late passage expressed higher levels of undifferentiated markers such as Oct4 and SSEA4 than those of early and middle passage. But differentiated cells of early and middle passage expressed higher level of differentiated state markers, Nestin (ectoderm), Brachyury (mesoderm), HNF3β (endoderm). From these results, it can be inferred that hESCs show higher proliferative abilities and reduced differentiation potentials as the passage number increased. Therefore, we conclude that early passage hESCs could be more suitable than middle and late passage hESCs in differentiation studies.
Related Links	http://dx.doi.org/10.3858/emm.2008.40.1.98
Starting Page	98
File Format	PDF
ISSN	12263613
Journal	Experimental & Molecular Medicine
Issue Number	1
Volume Number	40
Language	English
Publisher	Korean Society of Medical Biochemistry and Molecular Biology
Publisher Date	2008-01-01
Access Restriction	Open
Rights Holder	Korean Society of Medical Biochemistry and Molecular Biology
Subject Keyword	Clinical Biochemistry Molecular Medicine Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Molecular Biology Clinical Biochemistry Molecular Medicine

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