NDLI: Reactive Oxygen Species Facilitate Adipocyte Differentiation by Accelerating Mitotic Clonal Expansion*

Content Provider	PubMed Central
Author	Lee, Haemi Lee, Yoo Jeong Choi, Hyeonjin Ko, Eun Hee Kim, Jae-woo
Abstract	Growth-arrested 3T3-L1 preadipocytes rapidly express CCAAT/enhancer-binding protein-β (C/EBPβ) upon hormonal induction of differentiation. However, the DNA binding activity of C/EBPβ is not activated until the cells synchronously reenter S phase during the mitotic clonal expansion (MCE) phase of differentiation. In this period, C/EBPβ is sequentially phosphorylated by MAPK and glycogen synthase kinase-3β, inducing C/EBPβ DNA binding activity and transcription of its target genes. Because the DNA binding activity of C/EBPβ is further enhanced by oxidation in vitro, we investigated how redox state affects C/EBPβ DNA binding and MCE during adipogenesis. When 3T3-L1 cells were treated with H2O2 and hormonal stimuli, differentiation was accelerated with increased expression of peroxisome proliferator-activated receptor γ. Interestingly, cell cycle progression (S to G2/M phase) was markedly enhanced by H2O2, whereas antioxidants caused an S phase arrest during the MCE. H2O2 treatment resulted in the early appearance of a punctate pattern observed by immunofluorescent staining of C/EBPβ, which is a hallmark for C/EBPβ binding to regulatory elements, whereas a short antioxidant treatment rapidly dispersed the centromeric localization of C/EBPβ. Consistently, reactive oxygen species production was increased during 3T3-L1 differentiation. Our results indicate that redox-induced C/EBPβ DNA binding activity, along with the dual phosphorylation of C/EBPβ, is required for the MCE and terminal differentiation of adipocytes.
Related Links	http://dx.doi.org/10.1074/jbc.m808742200
Ending Page	10609
Page Count	9
Starting Page	10601
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	16
Volume Number	284
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2009-02-01
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Reactive Oxygen Species Facilitate Adipocyte Differentiation by Accelerating Mitotic Clonal Expansion

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