NDLI: Vaccination of Mice with a Yop Translocon Complex Elicits Antibodies That Are Protective against Infection with F1− Yersinia pestis ▿

Content Provider	PubMed Central
Author	Ivanov, Maya I. Noel, Betty L. Rampersaud, Ryan Mena, Patricio Benach, Jorge L. Bliska, James B.
Abstract	Yersinia pestis, the bacterial agent of plague, secretes several proteins important for pathogenesis or host protection. The F1 protein forms a capsule on the bacterial cell surface and is a well-characterized protective antigen but is not essential for virulence. A type III secretion system that is essential for virulence exports Yop proteins, which function as antiphagocytic or anti-inflammatory factors. Yop effectors (e.g., YopE) are delivered across the host cell plasma membrane by a translocon, composed of YopB and YopD. Complexes of YopB, YopD, and YopE (BDE) secreted by Yersinia pseudotuberculosis were purified by affinity chromatography and used as immunogens to determine if antibodies to the translocon could provide protection against Y. pestis in mice. Mice vaccinated with BDE generated high-titer immunoglobulin G antibodies specific for BDE, as shown by enzyme-linked immunosorbent assay and immunoblotting, and were protected against lethal intravenous challenge with F1− but not F1+ Y. pestis. Mice passively immunized with anti-BDE serum were protected from lethal challenge with F1− Y. pestis. The YopB protein or a complex of YopB and YopD (BD) was purified and determined by vaccination to be immunogenic in mice. Mice actively vaccinated with BD or passively vaccinated with anti-BD serum were protected against lethal challenge with F1− Y. pestis. These results indicate that anti-translocon antibodies can be used as immunotherapy to treat infections by F1− Y. pestis.
Related Links	http://dx.doi.org/10.1128/iai.00189-08
Ending Page	5190
Page Count	10
Starting Page	5181
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	11
Volume Number	76
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2008-11-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

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