NDLI: CE-microreactor-CE-MS/MS for protein analysis

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CE-microreactor-CE-MS/MS for protein analysis

Content Provider	PubMed Central
Author	Schoenherr, Regine M. Ye, Mingliang Vannatta, Michael Dovichi, Norman J.
Abstract	We present a proof-of-principle for a fully automated bottom-up approach to protein characterization. Proteins are first separated by capillary electrophoresis. A pepsin microreactor is incorporated into the distal end of this capillary. Peptides formed in the reactor are transferred to a second capillary, where they are separated by capillary electrophoresis and characterized by mass spectrometry. While peptides generated from one digestion are being separated in the second capillary, the next protein fraction undergoes digestion in the microreactor. The migration time in the first dimension capillary is characteristic of the protein while migration time in the second dimension is characteristic of the peptide. Spot capacity for the two-dimensional separation is 590. A MS/MS analysis of a mixture of cytochrome C and myoglobin generated Mascot MOWSE scores of 107 for cytochrome C and 58 for myoglobin. The sequence coverages were 48% and 22%, respectively.
Related Links	http://dx.doi.org/10.1021/ac061638h
Ending Page	2238
Page Count	9
Starting Page	2230
File Format	PDF
ISSN	00032700
e-ISSN	15206882
Journal	Analytical chemistry
Issue Number	6
Volume Number	79
Language	English
Publisher Date	2007-03-01
Access Restriction	Open
Subject Keyword	Analytical Chemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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