NDLI: Non-labeled QCM Biosensor for Bacterial Detection using Carbohydrate and Lectin Recognitions

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Non-labeled QCM Biosensor for Bacterial Detection using Carbohydrate and Lectin Recognitions

Content Provider	PubMed Central
Author	Shen, Zhihong Huang, Mingchuan Xiao, Caide Zhang, Yun Zeng, Xiangqun Wang, Peng G.
Abstract	High percentages of harmful microbes or their secreting toxins bind to specific carbohydrate sequences on human cells at the recognition and attachment sites. A number of studies also show that lectins react with specific structures of bacteria and fungi. In this report, we take advantage of the fact that a high percentage of microorganisms have both carbohydrate and lectin binding pockets at their surface. We demonstrate here for the first time that a carbohydrate non-labeled mass sensor in combination with lectin-bacterial O-antigen recognition can be used for detection of high molecular weight bacterial targets with remarkably high sensitivity and specificity. A functional mannose self-assembled monolayer (SAM) in combination with lectin Con A was used as molecular recognition elements for the detection of E. coli W1485 using Quartz Crytsal Microbalance (QCM) as a transducer. The multivalent binding of Concanavalin A (Con A) to the Escherichia coli (E. coli) surface O-antigen favors the strong adhesion of E. coli to mannose modified QCM surface by forming bridges between these two. As a result, the contact area between cell and QCM surface increases that leads to rigid and strong attachment. Therefore it enhances the binding between E. coli and the mannose. Our results show a significant improvement of the sensitivity and specificity of carbohydrate QCM biosensor with a experimental detection limit of a few hundred bacterial cells. The linear range is from 7.5 × 102 to 7.5 × 107 cells/mL that is four decade wider than the mannose alone QCM sensor. The change of damping resistances for E. coli adhesion experiments was no more than 1.4% suggesting that the bacterial attachment was rigid, rather than a viscoelastic behavior. Little non-specific binding was observed for Staphylococcus aureus and other proteins (Fetal Bovine serum, Erythrina cristagalli lectin). Our approach not only overcomes the challenges of applying QCM technology for bacterial detection but also increases the binding of bacteria to their carbohydrate receptor through bacterial surface binding lectins that significantly enhanced specificity and sensitivity of QCM biosensors. Combining carbohydrate and lectin recognition events with an appropriate QCM transducer can yield sensor devices highly suitable for the fast, reversible and straightforward on-line screening and detection of bacteria in food, water, clinical and biodefense areas.
Related Links	http://dx.doi.org/10.1021/ac061986j
Ending Page	2319
Page Count	8
Starting Page	2312
File Format	PDF
ISSN	00032700
e-ISSN	15206882
Journal	Analytical chemistry
Issue Number	6
Volume Number	79
Language	English
Publisher Date	2007-03-01
Access Restriction	Open
Subject Keyword	Analytical Chemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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