NDLI: Detection of Yersinia pestis in Sputum by Real-Time PCR

Content Provider	PubMed Central
Author	Caroline, Loïez Herwegh, Stéphanie Wallet, Frédéric Armand, Sylvie Guinet, Françoise Courcol, René J.
Copyright Year	2003
Abstract	A 5′ nuclease PCR assay for detection of the Yersinia pestis plasminogen activator (pla) gene in human respiratory specimens with simulated Y. pestis infection was developed. An internal positive control was added to the reaction mixture in order to detect the presence of PCR inhibitors that are often found in biological samples. The assay was 100% specific for Y. pestis. In the absence of inhibitors, a sensitivity of 102 CFU/ml of respiratory fluid was obtained. When inhibitors were present, detection of Y. pestis DNA required a longer sample treatment time and an initial concentration of bacteria of at least 104 CFU/ml. The test's total turnaround time was less than 5 h. The assay described here is well suited to the rapid diagnosis of pneumonic plague, the form of plague most likely to result from a bioterrorist attack.
Related Links	http://dx.doi.org/10.1128/jcm.41.10.4873-4875.2003
Ending Page	4875
Page Count	3
Starting Page	4873
File Format	PDF
ISSN	00951137
e-ISSN	1098660X
Journal	Journal of Clinical Microbiology
Issue Number	10
Volume Number	41
Language	English
Publisher	American Society for Microbiology
Publisher Date	2003-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Microbiology (medical) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology (medical)

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Detection of Yersinia pestis in Sputum by Real-Time PCR