NDLI: Primary Charge Separation in the Photosystem II Core from Synechocystis: A Comparison of Femtosecond Visible/Midinfrared Pump-Probe Spectra of Wild-Type and Two P680 Mutants

Content Provider	PubMed Central
Author	Donato, Mariangela Di Cohen, Rachel O. Diner, Bruce A. Breton, Jacques Van, Grondelle Rienk Groot, Marie Louise
Abstract	It is now quite well accepted that charge separation in PS2 reaction centers starts predominantly from the accessory chlorophyll BA and not from the special pair P680. To identify spectral signatures of BA, and to further clarify the process of primary charge separation, we compared the femtosecond-infrared pump-probe spectra of the wild-type (WT) PS2 core complex from the cyanobacterium Synechocystis sp. PCC 6803 with those of two mutants in which the histidine residue axially coordinated to PB (D2-His197) has been changed to Ala or Gln. By analogy with the structure of purple bacterial reaction centers, the mutated histidine is proposed to be indirectly H-bonded to the C9=O carbonyl of the putative primary donor BA through a water molecule. The constructed mutations are thus expected to perturb the vibrational properties of BA by modifying the hydrogen bond strength, possibly by displacing the H-bonded water molecule, and to modify the electronic properties and the charge localization of the oxidized donor \documentclass[10pt]{article}\usepackage{amsmath}\usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{pmc}\usepackage[Euler]{upgreek}\pagestyle{empty}\oddsidemargin -1.0in\begin{document}\begin{equation}{\mathrm{P}}_{680}^{+}.\end{equation}\end{document} Analysis of steady-state light-induced Fourier transform infrared difference spectra of the WT and the D2-His197Ala mutant indeed shows that a modification of the axially coordinating ligand to PB induces a charge redistribution of \documentclass[10pt]{article}\usepackage{amsmath}\usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{pmc}\usepackage[Euler]{upgreek}\pagestyle{empty}\oddsidemargin -1.0in\begin{document}\begin{equation}{\mathrm{P}}_{680}^{+}.\end{equation}\end{document} In addition, a comparison of the time-resolved visible/midinfrared spectra of the WT and mutants has allowed us to investigate the changes in the kinetics of primary charge separation induced by the mutations and to propose a band assignment identifying the characteristic vibrations of BA.
Related Links	http://dx.doi.org/10.1529/biophysj.107.122242
Starting Page	4783
File Format	PDF
ISSN	15420086
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	12
Volume Number	94
Language	English
Publisher	The Biophysical Society
Publisher Date	2008-06-15
Access Restriction	Open
Rights Holder	The Biophysical Society
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Charge Separation and Energy Transfer in the Photosystem II Core Complex Studied by Femtosecond Midinfrared Spectroscopy

Comparison of Primary Charge Separation in the Photosystem II Reaction Center Complex Isolated from Wild-type and D1-130 Mutants of the Cyanobacterium Synechocystis PCC 6803

Primary radical ion pairs in photosystem II core complexes

Femtosecond primary charge separation in Synechocystis sp. PCC 6803 photosystem I

Primary electron transfer processes in photosynthetic reaction centers from oxygenic organisms

Photosystem II activity of wild type Synechocystis PCC 6803 and its mutants with different plastoquinone pool redox states

Charge Separation, Stabilization, and Protein Relaxation in Photosystem II Core Particles with Closed Reaction Center

Purification and characterization of photosystem I and photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803.

A multimer model for P680, the primary electron donor of photosystem II.

Primary Charge Separation in the Photosystem II Core from Synechocystis: A Comparison of Femtosecond Visible/Midinfrared Pump-Probe Spectra of Wild-Type and Two P680 Mutants

Similar Documents

Charge Separation and Energy Transfer in the Photosystem II Core Complex Studied by Femtosecond Midinfrared Spectroscopy

Comparison of Primary Charge Separation in the Photosystem II Reaction Center Complex Isolated from Wild-type and D1-130 Mutants of the Cyanobacterium Synechocystis PCC 6803

Primary radical ion pairs in photosystem II core complexes

Femtosecond primary charge separation in Synechocystis sp. PCC 6803 photosystem I

Primary electron transfer processes in photosynthetic reaction centers from oxygenic organisms

Photosystem II activity of wild type Synechocystis PCC 6803 and its mutants with different plastoquinone pool redox states

Charge Separation, Stabilization, and Protein Relaxation in Photosystem II Core Particles with Closed Reaction Center

Purification and characterization of photosystem I and photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803.

A multimer model for P680, the primary electron donor of photosystem II.

Primary Charge Separation in the Photosystem II Core from Synechocystis: A Comparison of Femtosecond Visible/Midinfrared Pump-Probe Spectra of Wild-Type and Two P680 Mutants