NDLI: Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules

Content Provider	PubMed Central
Author	Yin, Hong Irina, Artsimovitch Landick, Robert Gelles, Jeff
Copyright Year	1999
Abstract	Cessation of transcription at specific terminator DNA sequences is used by viruses, bacteria, and eukaryotes to regulate the expression of downstream genes, but the mechanisms of transcription termination are poorly characterized. To elucidate the kinetic mechanism of termination at the intrinsic terminators of enteric bacteria, we observed, by using single-molecule light microscopy techniques, the behavior of surface-immobilized Escherichia coli RNA polymerase (RNAP) molecules in vitro. An RNAP molecule remains at a canonical intrinsic terminator for ≈64 s before releasing DNA, implying the formation of an elongation-incompetent (paused) intermediate by transcription complexes that terminate but not by those that read through the terminator. Analysis of pause lifetimes establishes a complete minimal mechanism of termination in which paused intermediate formation is both necessary and sufficient to induce release of RNAP at the terminator. The data suggest that intrinsic terminators function by a nonequilibrium process in which terminator effectiveness is determined by the relative rates of nucleotide addition and paused state entry by the transcription complex.
Starting Page	13124
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	23
Volume Number	96
Language	English
Publisher	The National Academy of Sciences
Publisher Date	1999-11-09
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Transcription. Comment on 'Mechanism of eukaryotic RNA polymerase III transcription termination'.

Transcription. Response to Comment on 'Mechanism of eukaryotic RNA polymerase III transcription termination'.

Attenuation in SV40 as a mechanism of transcription-termination by RNA polymerase B.

Mechanism of Eukaryotic RNA polymerase III transcription termination

Transcription termination by RNA polymerase III: uncoupling of polymerase release from termination signal recognition.

Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.

Termination of transcription by bacteriophage T3 RNA polymerase: homogeneous 3'-terminal oligonucleotide sequence of in vitro T3 RNA polymerase transcripts.

Mechanism of eukaryotic RNA polymerase III transcription termination.

Transcription termination factor La is also an initiation factor for RNA polymerase III.

Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules

Similar Documents

Transcription. Comment on 'Mechanism of eukaryotic RNA polymerase III transcription termination'.

Transcription. Response to Comment on 'Mechanism of eukaryotic RNA polymerase III transcription termination'.

Attenuation in SV40 as a mechanism of transcription-termination by RNA polymerase B.

Mechanism of Eukaryotic RNA polymerase III transcription termination

Transcription termination by RNA polymerase III: uncoupling of polymerase release from termination signal recognition.

Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.

Termination of transcription by bacteriophage T3 RNA polymerase: homogeneous 3'-terminal oligonucleotide sequence of in vitro T3 RNA polymerase transcripts.

Mechanism of eukaryotic RNA polymerase III transcription termination.

Transcription termination factor La is also an initiation factor for RNA polymerase III.

Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules