NDLI: An Epstein-Barr virus with a 58-kilobase-pair deletion that includes BARF0 transforms B lymphocytes in vitro.

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Interleukin-2 and alpha/beta interferon down-regulate hepatitis B virus gene expression in vivo by tumor necrosis factor-dependent and -independent pathways.

Herpes simplex virus type 1 DNA replication and gene expression during explant-induced reactivation of latently infected murine sensory ganglia.

Molecular analysis of herpes simplex virus type 1 during epinephrine-induced reactivation of latently infected rabbits in vivo.

Identification of Evi-3, a novel common site of retroviral integration in mouse AKXD B-cell lymphomas.

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Alterations of the p53 gene in Epstein-Barr virus-associated immunodeficiency-related lymphomas.

Nucleocapsid-glycoprotein interactions required for assembly of alphaviruses.

The presence of tat protein or tumor necrosis factor alpha is critical for herpes simplex virus type 1-induced expression of human immunodeficiency virus type 1.

The amino-terminal functions of the simian virus 40 large T antigen are required to overcome wild-type p53-mediated growth arrest of cells.

Neutralization sensitivity of human immunodeficiency virus type 1 is determined in part by the cell in which the virus is propagated.

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Sequence and genome organization of Borna disease virus.

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Inhibition of murine AIDS (MAIDS), development by the transplantation of bone marrow cells carrying the Fv-4 resistance gene to MAIDS virus-infected mice.

Down-regulation of HLA antigens by the adenovirus type 2 E3/19K protein in a T-lymphoma cell line.

An Epstein-Barr virus with a 58-kilobase-pair deletion that includes BARF0 transforms B lymphocytes in vitro.

Activities of the feline immunodeficiency virus integrase protein produced in Escherichia coli.

Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm.

Antigenic determinants of measles virus hemagglutinin associated with neurovirulence.

Neutralizing antibodies against hepatitis C virus and the emergence of neutralization escape mutant viruses.

Translational frameshifting at the gag-pol junction of human immunodeficiency virus type 1 is not increased in infected T-lymphoid cells.

Recombinant influenza virus polymerase: requirement of both 5' and 3' viral ends for endonuclease activity.

Spontaneous development of anti-hepatitis B virus envelope (anti-idiotypic) antibodies in animals immunized with human liver endonexin II or with the F(ab')2 fragment of anti-human liver endonexin II immunoglobulin G: evidence for a receptor-ligand-like relationship between small hepatitis B surface antigen and endonexin II.

Functional analysis of N-linked glycosylation mutants of the measles virus fusion protein synthesized by recombinant vaccinia virus vectors.

Transcription inhibition and other properties of matrix proteins expressed by M genes cloned from measles viruses and diseased human brain tissue.

Internal translation initiation on poliovirus RNA: further characterization of La function in poliovirus translation in vitro.

Influenza A virus M2 ion channel protein: a structure-function analysis.

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Induction of endogenous human cytomegalovirus gene expression after differentiation of monocytes from healthy carriers.

Minimal sequence requirements of a functional human immunodeficiency virus type 1 primer binding site.

Hormonal regulation of the gene for the type C ecotropic retrovirus receptor in rat liver cells.

Mucosal model of genital immunization in male rhesus macaques with a recombinant simian immunodeficiency virus p27 antigen.

Genetic analysis of the human immunodeficiency virus type 1 integrase protein.

Mapping a region of the large envelope protein required for hepatitis B virion maturation.

Enhanced replication of a hepatitis B virus mutant associated with an epidemic of fulminant hepatitis.

Nevirapine resistance mutations of human immunodeficiency virus type 1 selected during therapy.

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Mutations which affect the inhibition of protein phosphatase 2A by simian virus 40 small-t antigen in vitro decrease viral transformation.

Rearrangement of the VP6 gene of a group A rotavirus in combination with a point mutation affecting trimer stability.

Role of the matrix protein in the virion association of the human immunodeficiency virus type 1 envelope glycoprotein.

A Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, expressed after transformation of primary human B lymphocytes by Epstein-Barr virus (EBV) is induced by the EBV oncogene LMP1.

Identification and characterization of a human herpesvirus 6 gene segment capable of transactivating the human immunodeficiency virus type 1 long terminal repeat in an Sp1 binding site-dependent manner.

Cysteine residues in the Vif protein of human immunodeficiency virus type 1 are essential for viral infectivity.

Comparison of the effects of Sindbis virus and Sindbis virus replicons on host cell protein synthesis and cytopathogenicity in BHK cells.

Identification and characterization of a protein kinase gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus.

Multigenic evasion of inflammation by poxviruses.

An 85-kilodalton herpes simplex virus type 1 alpha trans-induction factor (VP16)-VP13/14 fusion protein retains the transactivation and structural properties of the wild-type molecule during virus infection.

Repression of enhancer II activity by a negative regulatory element in the hepatitis B virus genome.

Killer toxin-secreting double-stranded RNA mycoviruses in the yeasts Hanseniaspora uvarum and Zygosaccharomyces bailii.

Function of the cytoplasmic domain of a retroviral transmembrane protein: p15E-p2E cleavage activates the membrane fusion capability of the murine leukemia virus Env protein.

Viral multiplicity of attachment and its implications for human immunodeficiency virus therapies.

Antigenicity of the N8 influenza A virus neuraminidase: existence of an epitope at the subunit interface of the neuraminidase.

Splice site selection in polyomavirus late pre-mRNA processing.

Down-regulation of major histocompatibility complex class I synthesis by murine cytomegalovirus early gene expression.

Analysis of the signals for polarized transport of influenza virus (A/WSN/33) neuraminidase and human transferrin receptor, type II transmembrane proteins.

Mutational analysis of the conserved motifs of influenza A virus polymerase basic protein 1.

RAZ, an Epstein-Barr virus transdominant repressor that modulates the viral reactivation mechanism.

Role of oligomerization of the S13 Env-Sea oncoprotein in cell transformation.

Incomplete protection, but suppression of virus burden, elicited by subunit simian immunodeficiency virus vaccines.

Induction of HLA class I and class II expression in human T-lymphotropic virus type I-infected neuroblastoma cells.

Competitive quantitative PCR analysis of herpes simplex virus type 1 DNA and latency-associated transcript RNA in latently infected cells of the rat brain.

Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis.

Molecular evolution of herpesviruses: genomic and protein sequence comparisons.

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Sequence requirements of the Epstein-Barr virus latent origin of DNA replication.

Requirement of the Pr55gag precursor for incorporation of the Vpr product into human immunodeficiency virus type 1 viral particles.

Structure of intracellular mature vaccinia virus observed by cryoelectron microscopy.

Resistance of lymphocytic choriomeningitis virus to alpha/beta interferon and to gamma interferon.

Photoinactivation and kinetics of membrane fusion mediated by the human immunodeficiency virus type 1 envelope glycoprotein.

The phorbol ester phorbol myristate acetate inhibits human immunodeficiency virus type 1 envelope-mediated fusion by modulating an accessory component(s) in CD4-expressing cells.

Redesignation of the RNase D activity associated with retroviral reverse transcriptase as RNase H.

The activity of the pseudorabies virus latency-associated transcript promoter is dependent on its genomic location in herpes simplex virus recombinants as well as on the type of cell infected.

gp120-independent fusion mediated by the human immunodeficiency virus type 1 gp41 envelope glycoprotein: a reassessment.

Varicella-zoster virus open reading frame 4 protein is functionally distinct from and does not complement its herpes simplex virus type 1 homolog, ICP27.

Posttranscriptional effector domains in the Rev proteins of feline immunodeficiency virus and equine infectious anemia virus.

Functional role of the zipper motif region of human immunodeficiency virus type 1 transmembrane protein gp41.

Intermediates of adeno-associated virus DNA replication in vitro.

Characterization of human immunodeficiency virus type 1 variants with increased resistance to a C2-symmetric protease inhibitor.

Reduced nuclear import of human immunodeficiency virus type 1 preintegration complexes in the presence of a prototypic nuclear targeting signal.

Woodchuck hepatitis virus X protein is required for viral infection in vivo.

Upstream U3 sequences in simian immunodeficiency virus are selectively deleted in vivo in the absence of an intact nef gene.

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An Epstein-Barr virus with a 58-kilobase-pair deletion that includes BARF0 transforms B lymphocytes in vitro.

Content Provider	PubMed Central
Author	Robertson, E. S. Tomkinson, B. Kieff, E.
Abstract	A family of Epstein-Barr virus (EBV)-encoded RNAs found in nasopharyngeal carcinoma cells is also present at low levels in some latently infected and growth-transformed B lymphocytes (P. R. Smith, Y. Gao, L. Karran, M. D. Jones, D. Snudden, and B. E. Griffin, J. Virol. 67:3217-3225, 1993). A molecular genetic approach using EBV recombinants was undertaken to evaluate the role of these transcripts in primary B-lymphocyte growth transformation and latent infection. Since the se transcripts arise from a 22-kbp segment of the EBV genome and construction of large deletion mutants is an improbable result after transfection of infected cells with an EBV DNA fragment with a large deletion mutation, a new approach was taken to make a recombinant with the DNA encoding all of the BARF0 RNAs deleted. The approach derives from a recently described strategy for making recombinants from five overlapping EBV cosmid-cloned DNAs (B. Tomkinson, E. Robertson, R. Yalamanchili, R. Longnecker, and E. Kieff, J. Virol. 67:7298-7306, 1993). A large segment of EBV DNA was deleted from the transfected cosmid DNAs by omitting a cosmid which included all of the DNA encoding the BARF0 RNA and by ligating the distal halves of the two flanking cosmids so as to create one cosmid which had ends that overlapped with the other two unaltered cosmids. EBV recombinants with 58 kbp including BARF0 deleted resulted from transfecting the three overlapping EBV DNA fragments into P3HR-1 cells and simultaneously inducing lytic replication of the endogenous, transformation-defective, P3HR-1 EBV. The endogenous P3HR-1 EBV provided lytic infection and packaging functions. EBV recombinants with intact transforming functions were then selected by infecting primary B lymphocytes and growing the resultant transformed cells in lymphoblastoid cell lines. The efficiency of incorporation of the deletion into transforming EBV recombinants was close to that of a known indifferent marker, the type 1 EBNA 3A gene, indicating the absence of significant selection against the deletion. Cells infected with the deleted recombinant grew similarly to those infected with wild-type recombinants and had a similar level of permissiveness for lytic EBV infection. Thus, the BARF0 transcript is not critical to primary B-lymphocyte growth transformation or to latent infection. This methodology is useful for constructing EBV recombinants which are specifically mutated at other sites in the three cosmids and is a step toward deriving a minimal transforming EBV genome.
Starting Page	1449
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	3
Volume Number	68
Language	English
Publisher Date	1994-03-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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