NDLI: Cellular mechanisms of nitric oxide-induced relaxation of corporeal smooth muscle in the guinea-pig

Content Provider	PubMed Central
Author	Hikaru, Hashitani Fukuta, Hiroyasu Dickens, Emma J. Suzuki, Hikaru
Copyright Year	2002
Abstract	The cellular mechanism of nitric oxide (NO)-induced relaxation in corporeal smooth muscle (CSM) of the guinea-pig was investigated. Changes in the intracellular concentration of calcium ions ([Ca2+]i), membrane potential and isometric tension were measured. CSM cells exhibited spontaneous depolarizations and transient increases in [Ca2+]i (Ca2+ transients) which were accompanied by contractions. This spontaneous activity was abolished by nifedipine (10 μ m). NO released by 3-morpholino-sydnonimine (SIN-1, 10 μ m) hyperpolarized the membrane and prevented the generation of spontaneous depolarizations. SIN-1 also abolished Ca2+ transients and associated contractions. These effects of SIN-1 were blocked by 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ, 10 μ m), an inhibitor of guanylate cyclase. Noradrenaline (NA, 1 μ m) increased [Ca2+]i to levels similar to those produced by high potassium-containing solution (high K+ solution, [K+]o = 40 mm), however, NA-induced contractions were three times greater in amplitude than those induced by high K+ solution. In NA precontracted preparations, SIN-1 inhibited 80 % of the contraction and decreased [Ca2+]i by 20 %. In contrast, nifedipine reduced [Ca2+]i by 80 %, while the level of contraction was decreased by only 20 %. SIN-1-induced reduction in [Ca2+]i but not the tension effect, was abolished by pretreatment with cyclopiazonic acid (CPA, 10 μm). In high K+ precontracted preparations, SIN-1 inhibited 80 % of the contraction and reduced [Ca2+]i by 20 %. Nifedipine, however, largely abolished increases in both [Ca2+]i and tension under these circumstances. These results suggest that decreasing the sensitivity of contractile proteins to Ca2+ is probably the key mechanism of NO-induced relaxation in CSM of the guinea-pig.
Related Links	http://dx.doi.org/10.1113/jphysiol.2001.013049
Starting Page	573
File Format	PDF
ISSN	14697793
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	538
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2002-01-15
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mechanisms of relaxation induced by activation of β-adrenoreceptors in smooth muscle cells of the guinea-pig mesenteric artery

VIP-induced relaxation of guinea-pig intestinal smooth muscle cells: sequential involvement of cyclic AMP and nitric oxide.

VIP-induced relaxation of guinea-pig intestinal smooth muscle cells: sequential involvement of cyclic AMP and nitric oxide.

Relaxation mechanisms induced by stimulation of nerves and by nitric oxide in sheep urethral muscle.

Role of membrane potential in endothelium-dependent relaxation of guinea-pig coronary arterial smooth muscle.

Nitric oxide is involved in 5-HT-induced relaxations of the guinea-pig colon ascendens in vitro.

YC-1 potentiates nitric oxide-induced relaxation in guinea-pig trachea.

Spontaneous and noradrenaline-induced transient depolarizations in the smooth muscle of guinea-pig mesenteric vein.

Mechanisms of action of noradrenaline and carbachol on smooth muscle of guinea-pig anterior mesenteric artery.

Cellular mechanisms of nitric oxide-induced relaxation of corporeal smooth muscle in the guinea-pig

Similar Documents

Mechanisms of relaxation induced by activation of β-adrenoreceptors in smooth muscle cells of the guinea-pig mesenteric artery

VIP-induced relaxation of guinea-pig intestinal smooth muscle cells: sequential involvement of cyclic AMP and nitric oxide.

VIP-induced relaxation of guinea-pig intestinal smooth muscle cells: sequential involvement of cyclic AMP and nitric oxide.

Relaxation mechanisms induced by stimulation of nerves and by nitric oxide in sheep urethral muscle.

Role of membrane potential in endothelium-dependent relaxation of guinea-pig coronary arterial smooth muscle.

Nitric oxide is involved in 5-HT-induced relaxations of the guinea-pig colon ascendens in vitro.

YC-1 potentiates nitric oxide-induced relaxation in guinea-pig trachea.

Spontaneous and noradrenaline-induced transient depolarizations in the smooth muscle of guinea-pig mesenteric vein.

Mechanisms of action of noradrenaline and carbachol on smooth muscle of guinea-pig anterior mesenteric artery.

Cellular mechanisms of nitric oxide-induced relaxation of corporeal smooth muscle in the guinea-pig