NDLI: Analysis of whole-cell currents by patch clamp of guinea-pig myenteric neurones in intact ganglia

Content Provider	PubMed Central
Author	François, Rugiero Gola, Maurice Kunze, Wolf A. A. Reynaud, Jean-claude Furness, John B. Clerc, Nadine
Copyright Year	2002
Abstract	Whole-cell patch-clamp recordings taken from guinea-pig duodenal myenteric neurones within intact ganglia were used to determine the properties of S and AH neurones. Major currents that determine the states of AH neurones were identified and quantified. S neurones had resting potentials of −47 ± 6 mV and input resistances (R in) of 713 ± 49 MΩ at voltages ranging from −90 to −40 mV. At more negative levels, activation of a time-independent, caesium-sensitive, inward-rectifier current (I Kir) decreased R in to 103 ± 10 MΩ. AH neurones had resting potentials of −57 ± 4 mV and R in was 502 ± 27 MΩ. R in fell to 194 ± 16 MΩ upon hyperpolarization. This decrease was attributable mainly to the activation of a cationic h current, I h, and to I Kir. Resting potential and R in exhibited a low sensitivity to changes in [K+]o in both AH and S neurones. This indicates that both cells have a low background K+ permeability. The cationic current, I h, contributed about 20 % to the resting conductance of AH neurones. It had a half-activation voltage of −72 ± 2 mV, and a voltage sensitivity of 8.2 ± 0.7 mV per e-fold change. I h has relatively fast, voltage-dependent kinetics, with on and off time constants in the range of 50–350 ms. AH neurones had a previously undescribed, low threshold, slowly inactivating, sodium-dependent current that was poorly sensitive to TTX. In AH neurones, the post-action-potential slow hyperpolarizing current, I AHP, displayed large variation from cell to cell. I AHP appeared to be highly Ca2+ sensitive, since its activation with either membrane depolarization or caffeine (1 mm) was not prevented by perfusing the cell with 10 mm BAPTA. We determined the identity of the Ca2+ channels linked to I AHP. Action potentials of AH neurones that were elongated by TEA (10 mm) were similarly shortened and I AHP was suppressed with each of the three Ω-conotoxins GVIA, MVIIA and MVIIC (0.3–0.5 μm), but not with Ω-agatoxin IVA (0.2 μm). There was no additivity between the effects of the three conotoxins, which indicates the presence of N- but not of P/Q-type Ca2+ channels. A residual Ca2+ current, resistant to all toxins, but blocked by 0.5 mm Cd2+, could not generate I AHP. This patch-clamp study, performed on intact ganglia, demonstrates that the AH neurones of the guinea-pig duodenum are under the control of four major currents, I AHP, I h, an N-type Ca2+ current and a slowly inactivating Na+ current.
Related Links	http://dx.doi.org/10.1113/jphysiol.2001.013051
Ending Page	463
Page Count	17
Starting Page	447
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	538
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2002-01-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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