NDLI: Modulation of a presynaptic hyperpolarization-activated cationic current (I h) at an excitatory synaptic terminal in the rat auditory brainstem

Content Provider	PubMed Central
Author	Cuttle, Matthew F. Rusznák, Zoltán Wong, Adrian Y. C. Owens, Steven Forsythe, Ian D.
Copyright Year	2001
Abstract	A hyperpolarization-activated non-specific cation current, I h, was examined in bushy cell bodies and their giant presynaptic terminals (calyx of Held). Whole-cell patch clamp recordings were made using an in vitro brain slice preparation of the cochlear nucleus and the superior olivary complex. The aim was to characterise I h in identified cell bodies and synaptic terminals, to examine modulation by presynaptic cAMP and to test for modulatory effects of I h activation on synaptic transmission. Presynaptic I h was activated by hyperpolarizing voltage-steps, with half-activation (V 1/2) at –94 mV. Activation time constants were voltage dependent, showing an e-fold acceleration for hyperpolarizations of –32 mV (time constant of 78 ms at –130 mV). The reversal potential of I h was –29 mV. It was blocked by external perfusion of 1 mm CsCl but was unaffected by BaCl2. Application of internal cAMP shifted the activation curve to more positive potentials, giving a V 1/2 of –74 mV; hence around half of the current was activated at resting membrane potentials. This shift in half-activation was mimicked by external perfusion of a membrane-permeant analogue, 8-bromo-cAMP. The bushy cell body I h showed similar properties to those of the synaptic terminal; V 1/2 was –94 mV and the reversal potential was –33 mV. Somatic I h was blocked by CsCl (1 mm) and was partially sensitive to BaCl2. Somatic I h current density increased with postnatal age from 5 to 16 days old, suggesting that I h is functionally relevant during maturation of the auditory pathway. The function of I h in regulating presynaptic excitability is subtle. I h had little influence on EPSC amplitude at the calyx of Held, but may be associated with propagation of the action potential at branch points. Presynaptic I h shares properties with both HCN1 and HCN2 recombinant channel subunits, in that it gates relatively rapidly and is modulated by internal cAMP.
Related Links	http://dx.doi.org/10.1111/j.1469-7793.2001.00733.x
Ending Page	744
Page Count	12
Starting Page	733
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 3
Volume Number	534
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2001-08-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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