NDLI: Sarcoplasmic Reticulum Ca2+ Release Declines in Muscle Fibers from Aging Mice

Content Provider	PubMed Central
Author	Jiménez Moreno, Ramón Wang, Zhong-min Gerring, Robert C. Delbono, Osvaldo
Abstract	This study hypothesized that decline in sarcoplasmic reticulum (SR) Ca2+ release and maximal SR-releasable Ca2+ contributes to decreased specific force with aging. To test it, we recorded electrically evoked maximal isometric specific force followed by 4-chloro-m-cresol (4-CmC)-evoked maximal contracture force in single intact fibers from the mouse flexor digitorum brevis muscle. Significant differences in tetanic, but not in 4-CmC-evoked, contracture forces were recorded in fibers from aging mice as compared to younger mice. Peak intracellular Ca2+ in response to 4-CmC did not differ significantly. SR Ca2+ release was recorded in whole-cell patch-clamped fibers in the linescan mode of confocal microscopy using a low-affinity Ca2+ indicator (Oregon green bapta-5N) with high-intracellular ethylene glycol-bis(α-aminoethyl ether)-N,N,N′N′-tetraacetic acid (20 mM). Maximal SR Ca2+ release, but not voltage dependence, was significantly changed in fibers from old compared to young mice. Increasing the duration of fiber depolarization did not increase the maximal rate of SR Ca2+ release in fibers from old compared to young mice. Voltage-dependent inactivation of SR Ca2+ release did not differ significantly between fibers from young and old mice. These findings indicate that alterations in excitation-contraction coupling, but not in maximal SR-releasable Ca2+, account for the age-dependent decline in intracellular Ca2+ mobilization and specific force.
Related Links	http://dx.doi.org/10.1529/biophysj.107.118786
Starting Page	3178
File Format	PDF
ISSN	15420086
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	8
Volume Number	94
Language	English
Publisher	The Biophysical Society
Publisher Date	2008-04-15
Access Restriction	Open
Rights Holder	The Biophysical Society
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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