NDLI: Molecular and Biophysical Properties of Voltage-Gated Na+ Channels in Murine Vas Deferens

Content Provider	PubMed Central
Author	Zhu, Hai-lei Aishima, Manami Morinaga, Hidetaka Wassall, Richard D. Shibata, Atsushi Iwasa, Kazuomi Nomura, Masatoshi Nagao, Masaya Sueishi, Katsuo Cunnane, Thomas C. Teramoto, Noriyoshi
Abstract	The biological and molecular properties of tetrodotoxin (TTX)-sensitive voltage-gated Na+ currents (I Na) in murine vas deferens myocytes were investigated using patch-clamp techniques and molecular biological analyses. In whole-cell configuration, a fast, transient inward current was evoked in the presence of Cd2+, and was abolished by TTX (K d = 11.2 nM), mibefradil (K d = 3.3 μM), and external replacement of Na+ with monovalent cations (TEA+, Tris+, and NMDG+). The fast transient inward current was enhanced by veratridine, an activator of voltage-gated Na+ channels, suggesting that the fast transient inward current was a TTX-sensitive I Na. The values for half-maximal (V half) inactivation and activation of I Na were −46.3 mV and −26.0 mV, respectively. RT-PCR analysis revealed the expression of Scn1a, 2a, and 8a transcripts. The Scn8a transcript and the α-subunit protein of NaV1.6 were detected in smooth muscle layers. Using NaV1.6-null mice (NaV1.6−/−) lacking the expression of the Na+ channel gene, Scn8a, I Na were not detected in dispersed smooth muscle cells from the vas deferens, while TTX-sensitive I Na were recorded in their wild-type (NaV1.6+/+) littermates. This study demonstrates that the molecular identity of the voltage-gated Na+ channels responsible for the TTX-sensitive I Na in murine vas deferens myocytes is primarily NaV1.6.
Related Links	http://dx.doi.org/10.1529/biophysj.107.117192
Ending Page	3351
Page Count	12
Starting Page	3340
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	8
Volume Number	94
Language	English
Publisher	The Biophysical Society
Publisher Date	2008-04-01
Access Restriction	Open
Rights Holder	The Biophysical Society
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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