NDLI: Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Content Provider	PubMed Central
Author	Glick, Efrat Avi, Zrachya Levy, Yael Mett, Anahit Gidoni, David Eduard, Belausov Citovsky, Vitaly Gafni, Yedidya
Copyright Year	2007
Abstract	The V2 protein of tomato yellow leaf curl geminivirus (TYLCV) functions as an RNA-silencing suppressor that counteracts the innate immune response of the host plant. The host-cell target of V2, however, remains unknown. Here we show that V2 interacts directly with SlSGS3, the tomato homolog of the Arabidopsis SGS3 protein (AtSGS3), which is known to be involved in the RNA-silencing pathway. SlSGS3 genetically complemented an AtSGS3 mutation and restored RNA silencing, indicating that SlSGS3 is indeed a functional homolog of AtSGS3. A point mutant of V2 that is unable to bind SlSGS3 also lost its ability to suppress RNA silencing, suggesting a correlation between the V2–SlSGS3 interaction in planta and the suppressor activity of V2.
Related Links	http://dx.doi.org/10.1073/pnas.0709036105
Ending Page	161
Page Count	5
Starting Page	157
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	105
Language	English
Publisher	National Academy of Sciences
Publisher Date	2008-01-08
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein