NDLI: Vacuolar Storage Proteins Are Sorted in the Cis-Cisternae of the Pea Cotyledon Golgi Apparatus

Content Provider	PubMed Central
Author	Hillmer, Stefan Ali, Movafeghi Robinson, David G. Hinz, Giselbert
Copyright Year	2001
Abstract	Developing pea cotyledons contain functionally different vacuoles, a protein storage vacuole and a lytic vacuole. Lumenal as well as membrane proteins of the protein storage vacuole exit the Golgi apparatus in dense vesicles rather than in clathrin-coated vesicles (CCVs). Although the sorting receptor for vacuolar hydrolases BP-80 is present in CCVs, it is not detectable in dense vesicles. To localize these different vacuolar sorting events in the Golgi, we have compared the distribution of vacuolar storage proteins and of α-TIP, a membrane protein of the protein storage vacuole, with the distribution of the vacuolar sorting receptor BP-80 across the Golgi stack. Analysis of immunogold labeling from cryosections and from high pressure frozen samples has revealed a steep gradient in the distribution of the storage proteins within the Golgi stack. Intense labeling for storage proteins was registered for the cis-cisternae, contrasting with very low labeling for these antigens in the trans-cisternae. The distribution of BP-80 was the reverse, showing a peak in the trans-Golgi network with very low labeling of the cis-cisternae. These results indicate a spatial separation of different vacuolar sorting events in the Golgi apparatus of developing pea cotyledons.
Starting Page	41
File Format	PDF
ISSN	15408140
e-ISSN	15408140
Journal	The Journal of Cell Biology
Issue Number	1
Volume Number	152
Language	English
Publisher	The Rockefeller University Press
Publisher Date	2001-01-08
Access Restriction	Open
Rights Holder	The Rockefeller University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

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Vacuolar Storage Proteins Are Sorted in the Cis-Cisternae of the Pea Cotyledon Golgi Apparatus

Vacuolar storage proteins and the putative vacuolar sorting receptor BP-80 exit the golgi apparatus of developing pea cotyledons in different transport vesicles

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Vacuolar Storage Proteins Are Sorted in the Cis-Cisternae of the Pea Cotyledon Golgi Apparatus

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