NDLI: The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle

Content Provider	PubMed Central
Author	Kickhoefer, Valerie A. Liu, Yie Kong, Lawrence B. Snow, Bryan E. Stewart, Phoebe L. Harrington, Lea Rome, Leonard H.
Copyright Year	2001
Abstract	Vaults and telomerase are ribonucleoprotein (RNP) particles that share a common protein subunit, TEP1. Although its role in either complex has not yet been defined, TEP1 has been shown to interact with the mouse telomerase RNA and with several of the human vault RNAs in a yeast three-hybrid assay. An mTep1 −/− mouse was previously generated which resulted in no apparent change in telomere length or telomerase activity in six generations of mTep1-deficient mice. Here we show that the levels of the telomerase RNA and its association with the telomerase RNP are also unaffected in mTep1 − / − mice. Although vaults purified from the livers of mTep1−/− mice appear structurally intact by both negative stain and cryoelectron microscopy, three-dimensional reconstruction of the mTep1−/− vault revealed less density in the cap than previously observed for the intact rat vault. Furthermore, the absence of TEP1 completely disrupted the stable association of the vault RNA with the purified vault particle and also resulted in a decrease in the levels and stability of the vault RNA. Therefore, we have uncovered a novel role for TEP1 in vivo as an integral vault protein important for the stabilization and recruitment of the vault RNA to the vault particle.
Starting Page	157
File Format	PDF
ISSN	15408140
e-ISSN	15408140
Journal	The Journal of Cell Biology
Issue Number	1
Volume Number	152
Language	English
Publisher	The Rockefeller University Press
Publisher Date	2001-01-08
Access Restriction	Open
Rights Holder	The Rockefeller University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle

The La RNA-binding Protein Interacts with the Vault RNA and Is a Vault-associated Protein

The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle

The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle

Vaults and Telomerase Share a Common Subunit, TEP1

Assembly of Vault-like Particles in Insect Cells Expressing Only the Major Vault Protein

Telomerase-Associated Protein TEP1 Is Not Essential for Telomerase Activity or Telomere Length Maintenance In Vivo

Vault Poly(ADP-Ribose) Polymerase Is Associated with Mammalian Telomerase and Is Dispensable for Telomerase Function and Vault Structure In Vivo

Multiple Human Vault RNAs EXPRESSION AND ASSOCIATION WITH THE VAULT COMPLEX

The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle

Similar Documents

The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle

The La RNA-binding Protein Interacts with the Vault RNA and Is a Vault-associated Protein

The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle

The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle

Vaults and Telomerase Share a Common Subunit, TEP1

Assembly of Vault-like Particles in Insect Cells Expressing Only the Major Vault Protein

Telomerase-Associated Protein TEP1 Is Not Essential for Telomerase Activity or Telomere Length Maintenance In Vivo

Vault Poly(ADP-Ribose) Polymerase Is Associated with Mammalian Telomerase and Is Dispensable for Telomerase Function and Vault Structure In Vivo

Multiple Human Vault RNAs EXPRESSION AND ASSOCIATION WITH THE VAULT COMPLEX

The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle