NDLI: Alanine, not ammonia, is excreted from N2-fixingsoybean nodule bacteroids

Content Provider	PubMed Central
Author	Waters, James K. Hughes, Bobby L. Purcell, Larry C. Gerhardt, Klaus O. Mawhinney, Thomas P. Emerich, David W.
Copyright Year	1998
Abstract	Symbiotic nitrogen fixation, the process whereby nitrogen-fixingbacteria enter into associations with plants, provides the major sourceof nitrogen for the biosphere. Nitrogenase, a bacterial enzyme,catalyzes the reduction of atmospheric dinitrogen to ammonium. Inrhizobia-leguminous plant symbioses, the current model of nitrogentransfer from the symbiotic form of the bacteria, called a bacteroid,to the plant is that nitrogenase-generated ammonia diffuses across thebacteroid membrane and is assimilated into amino acids outside of thebacteroid. We purified soybean nodule bacteroids by a procedure thatremoved contaminating plant proteins and found that alanine was themajor nitrogen-containing compound excreted. Bacteroids incubated inthe presence of 15N2 excreted alanine highlyenriched in 15N. The ammonium in these assays neitheraccumulated significantly nor was enriched in 15N. Theresults demonstrate that a transport mechanism rather than diffusionfunctions at this critical step of nitrogen transfer from thebacteroids to the plant host. Alanine may serve only as a transportspecies, but this would permit physiological separation of thetransport of fixed nitrogen from other nitrogen metabolic functionscommonly mediated through glutamate.
Starting Page	12038
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	95
Language	English
Publisher	National Academy of Sciences
Publisher Date	1998-09-29
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Alanine dehydrogenase from soybean nodule bacteroids. Kinetic mechanism and pH studies.

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