NDLI: The kinetics and shear threshold of transient and rollinginteractions of L-selectin with its ligand on leukocytes

Content Provider	PubMed Central
Author	Alon, Ronen Chen, Shuqi Robert, Fuhlbrigge Puri, Kamal D. Springer, Timothy A.
Copyright Year	1998
Abstract	The kinetics of rolling and transient adhesions through selectinsmay depend on the kinetics and mechanical properties of theselectin:ligand bond, as well as on cellular properties includingreceptor-anchoring to the cell membrane and cytoskeleton. Kinetics areknown to depend on the selectin and may also be ligand dependent. Here,we study the kinetics of transient and rolling interactions ofleukocytes with L-selectin immobilized on a substrate. Remarkably, allproperties examined are similar to those seen when the sidedness isopposite, i.e., when the L-selectin ligand is on the substrate and whenthe ligand is isolated from HEV rather than present on leukocytes. Thesimilar properties include rolling velocity, a threshold shear stressabove 0.4 dyn/cm2 required to support rolling, ak°off of 7.0 to 6.8s−1 for the L-selectin tether bond, and a mechanical bondlength of 0.24 to 0.20 Å. Our results argue against a model in whichL-selectin shedding mediates rolling. Furthermore, the fast andforce-resistant kinetic properties suggest that L-selectin isspecialized dynamically for tethering leukocytes to vessel walls andadherent leukocytes.
Starting Page	11631
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	95
Language	English
Publisher	National Academy of Sciences
Publisher Date	1998-09-29
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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