NDLI: Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton

Content Provider	PubMed Central
Author	Burnett, Patrick E. Blackshaw, Seth Lai, Michael M. Qureshi, Irfan A. Burnett, Aaron F. Sabatini, David M. Snyder, Solomon H.
Copyright Year	1998
Abstract	p70 S6 kinase (p70S6k) is a mitogen-activated protein kinase that plays a central role in the control of mRNA translation. It physiologically phosphorylates the S6 protein of the 40s ribosomal subunit in response to mitogenic stimuli and is a downstream component of the rapamycin-sensitive pathway, which includes the 12-kDa FK506 binding protein and includes rapamycin and the 12-kDa FK506 binding protein target 1. Here, we report the identification of neurabin (neural tissue-specific F-actin binding protein), a neuronally enriched protein of 1,095 amino acids that contains a PDZ domain and binds p70S6k. We demonstrate the neurabin-p70S6k interaction by yeast two-hybrid analysis and biochemical techniques. p70S6k and neurabin coimmunoprecipitate from transfected HEK293 cells. Site-directed mutagenesis of neurabin implicates its PDZ domain in the interaction with p70S6k, and deletion of the carboxyl-terminal five amino acids of p70S6k abrogates the interaction. Cotransfection of neurabin in HEK293 cells activates p70S6k kinase activity. The mRNA of neurabin and p70S6k show striking colocalization in brain sections by in situ hybridization. Subcellular fractionation of rat brain demonstrates that neurabin and p70S6k both localize to the soluble fraction of synaptosomes. By way of its PDZ domain, the neuronal-specific neurabin may target p70S6k to nerve terminals.
Starting Page	8351
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	14
Volume Number	95
Language	English
Publisher	The National Academy of Sciences
Publisher Date	1998-07-07
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton

Interleukin 2 stimulation of p70 S6 kinase activity is inhibited by the immunosuppressant rapamycin.

p70 S6 Kinase Is Regulated by Protein Kinase Cζ and Participates in a Phosphoinositide 3-Kinase-Regulated Signalling Complex

Phosphatidylinositol 3-kinase signals activation of p70 S6 kinase in situ through site-specific p70 phosphorylation.

Glycogen synthase kinase (GSK)-3 promotes p70 ribosomal protein S6 kinase (p70S6K) activity and cell proliferation

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RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1

A single gene encodes two isoforms of the p70 S6 kinase: activation upon mitogenic stimulation.

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Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton

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Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton

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