NDLI: Identification of yeast proteins necessary for cell-surface function of a potassium channel

Content Provider	PubMed Central
Author	Haass, Friederike A. Martin, Jonikas Walter, Peter Weissman, Jonathan S. Jan, Yuh-nung Jan, Lily Y. Schuldiner, Maya
Copyright Year	2007
Abstract	Inwardly rectifying potassium (Kir) channels form gates in the cell membrane that regulate the flow of K+ ions into and out of the cell, thereby influencing the membrane potential and electrical signaling of many cell types, including neurons and cardiomyocytes. Kir-channel function depends on other cellular proteins that aid in the folding of channel subunits, assembly into tetrameric complexes, trafficking of quality-controlled channels to the plasma membrane, and regulation of channel activity at the cell surface. We used the yeast Saccharomyces cerevisiae as a model system to identify proteins necessary for the functional expression of a mammalian Kir channel at the cell surface. A screen of 376 yeast strains, each lacking one nonessential protein localized to the early secretory pathway, identified seven deletion strains in which functional expression of the Kir channel at the plasma membrane was impaired. Six deletions were of genes with known functions in trafficking and lipid biosynthesis (sur4Δ, csg2Δ, erv14Δ, emp24Δ, erv25Δ, and bst1Δ), and one deletion was of an uncharacterized gene (yil039wΔ). We provide genetic and functional evidence that Yil039wp, a conserved, phosphoesterase domain-containing protein, which we named “trafficking of Emp24p/Erv25p-dependent cargo disrupted 1” (Ted1p), acts together with Emp24p/Erv25p in cargo exit from the endoplasmic reticulum (ER). The seven yeast proteins identified in our screen likely impact Kir-channel functional expression at the level of vesicle budding from the ER and/or the local lipid environment at the plasma membrane.
Related Links	http://dx.doi.org/10.1073/pnas.0708765104
Ending Page	18084
Page Count	6
Starting Page	18079
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	46
Volume Number	104
Language	English
Publisher	National Academy of Sciences
Publisher Date	2007-11-13
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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